Edaravone regulates Sirt3 expression in lipopolysaccharide-activated microglia via MAPKs signaling pathway
Objective To investigate the effect of edaravone on Sirt3 and MAPKs pathway-related proteins in LPS-activated microglia and its regulatory mechanism.Methods Western Blot and immunofluorescence were used to detect the effect of edaravone on the protein expression of Sirt3 and MAPKs pathway-related proteins ERK1/2,P38,JNK as well as their phosphorylated forms p-ERK1/2,p-P38 and p-JNK in the activated BV2 cells.Western Blot techniques were applied to detect the effects of ERK1/2 pathway inhibitor on the expressions of p-ERK1/2 and Sirt3.Results The expressions of Sirt3,p-ERK1/2,p-P38 and p-JNK in microglia were significantly increased after BV2 cells activation.Edaravone promoted the expression of Sirt3 and p-ERK1/2 and downregulated the expression of p-JNK and p-P38.The protein expression of Sirt3 and p-ERK1/2 decreased after co-treatment with edaravone and ERK1/2 inhibitor.Edaravone promoted the overexpression of Sirt3 and p-ERK1/2 and downregulated the expression of p-JNK and p-P38.Conclusions Edaravone can regulate MAPKs signaling pathway and promote Sirt3 production in BV2 cells,possibly regulates neuroinflammatory responses via ERK/Sirt3 signaling pathway.
万方数据
维普
