Protective effect of Baicalin on rats with pulmonary hypertension through PDGF/P38 MAPK signaling pathway
Objective To investigate the protective effect and mechanism of Baicalin on monocrotaline-induced pulmonary hypertension in rats.Methods Forty SD rats were randomly divided into 4 groups:a control group,a pulmonary hypertension group,a baicalin 50 mg·kg-1 group and a baicalin 100 mg·kg-1 group.A single intraperitoneal injection of monocrotaline(60mg/kg)was used to establish the pulmonary hypertension model.After injection of monocrotaline,baicalin was gavage continuously for 28 days.Right ventricular catheterization method was used to detect RVSP and mPAP.The levels of SOD,GSH-px,ICAM-1,VCAM-1,IL-8 and IL-1β in serum were detected by ELISA.The formation of reactive oxygen species(ROS)in lung tissue was observed by DHE staining.The expression of bax and bcl-2 in lung tissue were detected by immunofluorescence.Western blot was used to detect the protein expression of PDGF and P38 MAPK.Results Compared with the model group,mPAP,RVSP,RVHI and W/D were significantly decreased,and the contents of ICAM-1,VCAM-1,IL-8 and IL-1β in serum were significantly decreased,the contents of bcl-2,SOD and GSH-px were increased significantly in the Baicalin group.The contents of ROS,bax,PDGF and P38 MAPK and the level of bax/bcl-2 ratio in lung tissue were significantly decreased in the Baicalin group.Conclusions Baicalin may play a protective role in improving inflammation,oxidative stress and apoptosis of rats with pulmonary hypertension through PDGF/P38 MAPK signaling pathway.
万方数据
维普
