Inhibition effects of cannabidiol on inflammatory response following multiple cerebral concussions in rats
Objective To investigate the mechanism by which cannabidiol(CBD)inhibits neuro-inflammation and protects neural tissue in a rat model of multiple cerebral concussions.Methods Rat models of multiple cerebral concussions(MCC)were established,and Hematoxylin-eosin(HE)and immunohistochemical staining were used to assess changes in neurons and microglial cells after multiple cerebral concussions and the protective effects of CBD.Western blot was performed to measure the expression of microglial cell marker Iba-1 and pro-inflammatory factors IL-1β and TNFα in different brain regions.Results HE staining revealed significant pathological changes in neurons following MCC in rats compared to the Sham group.CBD intervention led to a restoration of normal neuronal morphology,with a more pronounced effect observed in the high-dose group.Immunohistochemical staining showed that microglial cells in the Sham group were in a resting state,while their numbers increased and exhibited an activated state after MCC.CBD intervention resulted in a shift of microglial cells towards a resting state,with a more significant effect observed in the high-dose group.Western blot demonstrated a significant upregulation of Iba-1,IL-1β,and TNFα protein levels in different brain regions after MCC(P＜0.05).CBD intervention led to a downregulation of Iba-1,IL-1β,and TNFα expression in these brain regions(P＜0.05).Conclusions Cannabidiol could alleviate inflammatory response in the cortex and hippocampus of rats with multiple cerebral concussions and exhibit neuroprotective effects.
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