Objective To explore the expression of Polo-like kinase 2(PLK2)in glioblastoma(GBM)tissues and its clinical significance.Methods The TCGA database was retrieved to obtain the clinical data regarding the expression of PLK2 in 163 GBM tissues and 207 normal brain tissues,and subsequently,the expression of PLK2 was analyzed.The UALCAN database was consulted to analyze the methylation level of the PLK1 promoter.The mRNA level of PLK2 was detected by RT-PCR in the GBM tissues obtained from 50 GBM patients who underwent surgery from May 2019 to June 2022 and in the non-tumor brain tissues resected during decompression in 20 patients with traumatic brain injury;the methylation status of the PLK2 promoter was analyzed by the MSP method;concurrently,the relationship between the expression level of PLK1 mRNA in GBM tissues and the overall survival(OS)of patients was analyzed.Results The TCGA database analysis indicated that the expression level of PLK2 in GBM tissues was significantly lower than that in normal cerebral tissues(P<0.05),and the median OS of GBM patients in the high-expression group of PLK2 was significantly lower than that in the low-expression group(P<0.05).The UALCAN database analysis disclosed that the methylation level of the PLK2 promoter in GBM tissues was elevated compared to that in normal cerebral tissues(P<0.001).The expression level of PLK2 mRNA in 50 GBM tissue specimens was strikingly lower than that in the control group(P<0.001).Based on the median value of PLK2 mRNA expression in GBM tissues,50 GBM patients were categorized into the high-expression group(n=20)and the low-expression group(n=30).Multivariate Cox regression analysis demonstrated that high expression of PLK2 mRNA was an independent risk factor for the unfavorable survival prognosis of GBM patients(P<0.05).The Kaplan-Meier curve revealed that the median OS of GBM patients with low expression of PLK2 mRNA was longer(15.5 months vs.10.0 months;P<0.001).The expression level of PLK2 mRNA in GBM tissues with hypermethylation of PLK2 was even lower(P<0.001).Conclusions PLK2 is expressed at a relatively low level in GBM tissues,which might be regulated by the methylation of its DNA promoter.The lower expression of PLK2 in GBM tissues could potentially serve as an independent prognostic factor for GBM patients.
维普
万方数据