摘要
目的 探讨血浆差异表达微小RNA(miRNA)在预测脑膜瘤术后复发中的作用.方法 2017年5～9月前瞻性收集5例脑膜瘤和5例健康体检者的血浆样本,采用TaqMan低密度阵列(TLDA)分析鉴定差异表达的miRNA.2017年11月至2023年11月前瞻性收集脑膜瘤97例,术前PCR检测血浆差异表达miRNA,末次随访复查MRI判断肿瘤复发情况.结果 TLDA分析共鉴定出9个差异表达miRNA(miR-21、miR-219-5p、miR-375、miR-190a、miR-451、miR-224、miR-107、miR-539、miR-197).与未复发脑膜瘤病人比较,复发脑膜瘤病人血浆miR-21、miR-219-5p、miR-375、miR-190a表达量显著升高(P<0.05),血浆miR-451、miR-224、miR-107、miR-539、miR-197表达量显著降低(P<0.05).末次随访,15例(15.46％)出现复发.多因素Cox回归分析显示,血浆miR-21、miR-219-5p、miR-190a高表达以及血浆miR-107、miR-539、miR-224低表达均与脑膜瘤术后复发密切相关(P<0.05).Kaplan-Meier曲线分析显示,血浆miR-21≥2.64、miR-219-5p≥2.72、miR-190a≥1.82以及血浆miR-107≤3.78、miR-539≤4.69、miR-224≤4.59病人肿瘤无复发生存时间明显缩短(P<0.05).结论 脑膜瘤病人差异表达血浆miR-21、miR-219-5p、miR-375、miR-190a、miR-451、miR-107、miR-539、miR-224、miR-197,监测这些miRNA可辅助预测脑膜瘤术后复发风险.
Abstract
Objective To explore the role of differentially expressed microRNAs (miRNAs) in plasma in predicting postoperative recurrence of meningiomas. Methods Plasma samples of 5 patients with meningiomas and 5 healthy subjects were prospectively collected from May to September 2017. TaqMan Low-Density Array (TLDA) analysis was used to identify differentially expressed miRNAs. Ninty-seven patients with meningiomas were prospectively collected from November 2017 to November 2023. Preoperative PCR was used to detect differentially expressed miRNAs in plasma,and tumor recurrence was determined by MRI at the last follow-up. Results A total of 9 differentially expressed miRNAs (miR-21,miR-219-5p,miR-375,miR-190a,miR-451,miR-224,miR-107,miR-539,miR-197) were identified by TLDA analysis. Compared with patients with non-recurrent meningiomas,the expression levels of miR-21,miR-219-5p,miR-375,and miR-190a in the plasma of patients with recurrent meningiomas were significantly increased (P<0.05),while the expression levels of miR-451,miR-224,miR-107,miR-539,and miR-197 in the plasma were significantly decreased (P<0.05). At the last follow-up,15 patients (15.46％) had recurrence. Multivariate Cox regression analysis indicated that,high levels of miR-21,miR-219-5p,and miR-190a in plasma,and low levels of miR-107,miR-539,and miR-224 in plasma were closely related to postoperative recurrence of meningiomas (P<0.05). Kaplan-Meier curve analysis showed that patients with plasma miR-21≥2.64,miR-219-5p ≥2.72,miR-190a ≥1.82,and plasma miR-107 ≤3.78,miR-539 ≤4.69,and miR-224 ≤4.59 had significantly shorter recurrence-free survival time (P<0.05). Conclusion Patients with meningiomas have differentially expressed plasma miR-21,miR-219-5p,miR-375,miR-190a,miR-451,miR-107,miR-539,miR-224,miR-197,and miR-190a,and monitoring these miRNAs can assist in predicting the risk of postoperative recurrence of meningiomas.
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