Objective To investigate the value of serum soluble human leucine-rich repeats and immunoglobulin-like domain 3 (sLRIG3) in predicting prognosis of patients with glioblastoma multiforme (GBM).Methods A total of 60 patients with GBM were prospectively enrolled from September 2019 to December 2022,and their tumor tissues and serum samples were collected for analysis.Additionally,serum samples from 60 healthy individuals served as control.The serum sLRIG3 level was tested using enzyme-linked immunosorbent assay (ELISA),and LRIG3 expression in tumor tissues was assessed by immunohistochemistry.The follow-up period for GBM patients extended until either death or December 31,2023,during which progression-free survival (PFS) and overall survival (OS) data were recorded.Results The mean serum sLRIG3 level in GBM patients (2.84±0.89 ng/ml) was significantly lower than that (4.62±2.16 ng/ml) in the control group (P<0.001).Immunohistochemical staining revealed that high-expression of LRIG3 was observed in 38 GBM patients (63.33％),while low-expression in the remaining 22 GBM patients.The serum sLRIG3 levels in GBM patients with high-expression of LRIG3 (1.98±0.33 ng/ml) was significantly lower than that (3.33±0.72 ng/ml) in GBM patients with low-expression of LRIG3 (P<0.001).The 60 GBM patients were followed up for 12～48 months.The median PFS was 12.50 months (IQR:8.78～21.30 months),and the median OS was 16.40 months (IQR:10.13～24.70 months).The serum sLRIG3 level of GBM patients with tumor progression (2.74±0.84 ng/ml) was significantly lower than that (3.92±0.72 ng/ml) of patients without tumor progression (P=0.004).The serum sLRIG3 level of dead GBM patients (2.42±0.71 ng/ml) was significantly lower than that (3.47±0.76 ng/ml) of surviving patients (P<0.001).The multivariate Cox proportional hazards regression model analysis demonstrated that a decreased serum sLRIG 3 level was an independent predictive factor for the death of GBM patients.The Kaplan-Meier curve analysis indicated that the median OS of GBM patients with a serum sLRIG3 level<3.23 ng/ml was significantly shortened (P=0.016).Conclusion The serum sLRIG3 level of GBM patients is significantly decreased and is closely associated with the poor prognosis of the patients.
关键词
胶质母细胞瘤/可溶性人类富含亮氨酸重复序列和免疫球蛋白样结构域3(sLRIG3)/血清/生存预后
Key words
Glioblastoma multiforme/Soluble human leucine-rich repeats and immunoglobulin-like domain 3 (sLRIG3)/Serum/Survival prognosis
维普
万方数据