Objective To explore the expression,function and regulatory mechanism of integrin subunit beta 2 (ITGB2) in glioma.Methods The expressions of ITGB2 mRNA in glioma tissue and adjacent non-tumor brain tissue samples which were collected from 25 patients with glioma undergoing surgical resection from June 2018 to June 2019 was detected by real-time quantitative PCR.Glioma cell lines (A172,T98G,U138-MG) were cultured in vitro.E2F2 and ITGB2 small interfering RNAs were transfected to regulate the gene expression of cells.Cell proliferation was detected by CCK-8 assay and EdU staining,and cell cycle distribution was detected by flow cytometry.The binding relationship between E2F2 and the promoter region of ITGB2 was verified by dual luciferase reporter gene assay,and the influence of E2F2 on the expression of ITGB2 protein was detected by Western blotting.Results Compared with the non-tumor brain tissues adjacent to the tumor,the expression of ITGB2 mRNA in glioma tissues was significantly upregulated.Knockdown of ITGB2 expression inhibited the proliferation of glioma cells in vitro and induced cell cycle arrest.E2F2 bound to the promoter region of ITGB2.Overexpression of E2F2 promoted the expression of ITGB2 protein,and knockdown of E2F2 inhibited the expression of ITGB2 protein.Overexpression of E2F2 reversed the inhibitory effect of knockdown of ITGB2 on the growth of glioma cells.Conclusion ITGB2 is highly expressed in glioma tissues and promotes glioma cell proliferation,and its high expression is regulated by E2F2.
维普
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