摘要
目的 研究意大利牛舌草提取物对咳嗽变异性哮喘(CVA)的作用,及其作用机制.方法 将48只SD大鼠随机分为空白组、模型组、对照组和实验A1、A5、A7组,每组8只.除空白组外,其余各组用卵蛋白-完全弗氏佐剂进行造模.造模成功后,空白组与模型组均灌胃给予等量0.9％NaCl;对照组灌胃给予250 mg·kg-1醋酸泼尼松;实验A1、A5、A7组分别灌胃给予61.1 mg·kg-1意大利牛舌草提取物A1、48.8 mg·kg-1意大利牛舌草提取物A5、201.8 mg·kg-1意大利牛舌草提取物A7,连续灌胃28 d.观察各组大鼠的咳嗽次数,用蛋白质印迹法检测各组大鼠肺组织中Toll样受体4(TLR4)、髓样分化因子(MyD88)和核因子κB(NF-κB)蛋白质表达的变化.结果 空白组、模型组、对照组和实验A1、A5、A7 组的咳嗽次数分别为(1.10±0.22)、(8.33±1.24)、(3.08±0.65)、(3.31±0.99)、(3.08±1.02)和(3.06±0.68)次,TLR4 蛋白相对表达水平分别为 0.61±0.01、0.84±0.04、0.66±0.02、0.64±0.04、0.64±0.03 和0.69±0.02,MyD88 蛋白相对表达水平分别为 0.54±0.08、0.86±0.06、0.71±0.06、0.65±0.05、0.64±0.08 和 0.70±0.06,p-NF-κB 蛋白相对表达水平分别为 0.48±0.11、0.94±0.06、0.80±0.08、0.68±0.04、0.68±0.06 和0.78±0.09.模型组的上述指标与实验A1、A5、A7组、对照组和正常组相比,在统计学上差异均有统计学意义(均P＜0.05).结论 意大利牛舌草提取物A1、A5、A7对大鼠的CVA具有抑制作用,其作用机制可能与抑制TLR4/MyD88/NF-κB信号通路有关.
Abstract
Objective To investigate the effect of Anchusa italica Retz.extract on cough variant asthma(CVA)and its mechanism of action.Methods Forty-eight SD rats of each eight were randomly divided into blank group(equal amount of saline),model group(equal amount of saline),control group(250 mg·kg-1 prednisone acetate)and experimental-A1,-A5,-A7 groups(61.1 mg·kg-1 Anchusa italica Retz.extract A1,48.8 mg·kg-1 Anchusa italica Retz.extract A5,201.8 mg·kg-1 Anchusa italica Retz.extract A7),continuous gavage for 28 d;except for the blank group,the other groups were made with ovalbumin-complete Freund's adjuvant.The number of cough times of each rat was observed.The changes in the expression of Toll-like receptor 4(TLR4),myeloid differentiation factor(MyD88)and nuclear factor κB(NF-κB)proteins were observed in the lung tissue.Results The cough times in the blank group,model group,control group and experimental-A1,-A5,-A7 groups were 1.10±0.22,8.33±1.24,3.08±0.65,3.31±0.99,3.08±1.02 and 3.06±0.68;the relative expression levels of TLR4 protein were 0.61±0.01,0.84±0.04,0.66±0.02,0.64±0.04,0.64±0.03 and 0.69±0.02;the relative expression levels of MyD88 protein were 0.54±0.08,0.86±0.06,0.71±0.06,0.65±0.05,0.64±0.08 and 0.70±0.06;the relative expression levels of p-NF-κB protein were 0.48±0.11,0.94±0.06,0.80±0.08,0.68±0.04,0.68±0.06 and 0.78±0.09.Compared with the experimental-A1,-A5,-A7 groups,control group,normal group,the differences were statistically significant in the model group(all P＜0.05).Conclusion The extract of Anchusa Italica Retz.can inhibit the symptoms of CVA rats,and the mechanism may be related to the inhibition of TLR4/MyD88/NF-κB signaling pathway.
基金项目
新疆维吾尔自治区自然科学基金项目联合基金面上项目(2021D01C222)
新疆医科大学省部共建中亚高发病成因与防治国家重点实验室开放课题基金资助项目(SKL-HIDCA-2020-ZY12)
新疆地产中药民族药新药研发培育基金资助项目(2017-02-08)
国家科技期刊平台
NSTL
万方数据