Protective effect of platycodon platycodon D on renal injury induced by exhaustive exercise in rats
Objective To analyze the protective effect of platycodon platycodon D on renal injury induced by exercise exhaustion in rats.Methods The model of kidney injury induced by exhaustive exercise was established by treadmill training.Fifty SD rats were divided into control group(0.9％NaCl),model group(exhaustive exercise model),experimental-L,-M,-H groups(25,50,100 mg·kg-1 platycodon D).Renal function[blood urea nitrogen(BUN),serum creatinine(Scr)],renal injury markers[neutrophil gelatinase associated lipocalin(NGAL),kidney injury molecule-1(KIM-1)]were detected by the kit;nucleotide oligomeric domain-like receptor family 3(NLRP3),apoptosis-associated speck-like protein containing a card(ASC),cysteine aspartic acid specific protease-1(Caspase-1)protein expressions were detected by Western blot.Results BUN levels in control group,model group,experimental-L,-M,-H groups were(5.52±0.49),(9.98±0.85),(8.30±0.65),(7.12±0.47)and(5.98±0.63)mmol·L-1;Scr were(1.69±0.24),(5.69±0.67),(4.71±0.47),(3.55±0.25)and(2.67±0.24)ng·mL-1;NGAL were(207.58±28.56),(613.42±63.87),(521.52±55.35),(419.89±40.85)and(278.13±26.48)pg·mL-1;KIM-1 were(12.11±1.52),(80.42±9.56),(69.03±7.58),(47.32±6.67)and(24.67±3.60)ng·L-1;NLRP3 protein expression were 0.43±0.06,1.35±0.11,1.03±0.10,0.78±0.09 and 0.53±0.06;ASC protein expression were 0.25±0.03,0.74±0.06,0.60±0.06,0.44±0.05 and 0.31±0.04;Caspase-1 protein expression were 0.31±0.04,0.83±0.07,0.69±0.06,0.53±0.05 and 0.42±0.04.Compared with the control group,the above indexes in the model group were statistically significant(all P＜0.01);compared with the model group,the above indexes of the experimental-L,-M,-H groups were statistically significant(all P＜0.01).Conclusion Platycodon D can inhibit the activation of NLRP3 inflammasome to alleviate the renal injury induced by exhaustive exercise in rats.
国家科技期刊平台
NSTL
万方数据
