基于Ⅱ型胶原诱导性关节炎模型大鼠探讨雷公藤多苷片的长期毒性研究
Study on long-term toxicity of Tripterygium glycosides tablets based on type Ⅱ collagen-induced arthritis model rats
叶田香 1石明珠 1杨卫东 1魏砚明 1李会芳 1程生辉2
作者信息
- 1. 山西中医药大学中药与食品工程学院,山西晋中 030619
- 2. 山西中医药大学基础医学院,山西晋中 030619
- 折叠
摘要
目的 观察雷公藤多苷片对Ⅱ型胶原诱导性关节炎(CIA)大鼠多系统的毒性反应.方法 将健康SD大鼠随机分为正常组、模型组及实验组,每组10只;除正常组外,其余各组建立胶原诱导性关节炎模型.首次免疫后灌胃给药,正常组和模型组均给予等体积0.3％羧甲基纤维素钠溶液,实验组给予72 mg·kg-1·d-1雷公藤多苷溶液,每天给药1次,连续给药6周.于第42天取材,各组分别检测血常规,计算脏器指数;用酶联免疫吸附试验法检测大鼠肝、肾功能及性激素等水平;用苏木精-伊红(HE)染色法观察肝、肾、卵巢、睾丸组织病理形态学改变.结果 正常组、模型组和实验组的睾丸指数分别为(0.81±0.05)％、(0.97±0.06)％和(0.81±0.12)％,雌二醇含量分别为(63.90±16.19)、(55.42±7.23)和(40.04±5.97)pg·mL-1,睾酮含量分别为(1 290.96±257.94)、(1 198.43±190.77)和(912.62±61.72)pg·mL-1.模型组的上述指标与实验组比较,在统计学上差异均有统计学意义(P＜0.01,P＜0.05)o HE病理结果表明,雷公藤多苷片能引起CIA模型大鼠的卵巢、睾丸组织病理学异常改变.结论 雷公藤多苷片8倍临床等效剂量连续用药6周可以引起CIA大鼠生殖系统的损伤.
Abstract
Objective To observe the multi-system toxicity of Tripterygium glycosides tablets in rats with type Ⅱ collagen-induced arthritis(CIA).Methods Healthy SD rats were randomly divided into normal group,model group and experimental group,with 10 rats in each group.In addition to the normal group,the other groups were established collagen-induced arthritis model.After the first immunization,the normal group and the model group were given an equal volume of 0.3％sodium carboxymethyl cellulose solution,and the experimental group was given 72 mg·kg-1·d-1 Tripterygium glycosides solution,once a day for 6 weeks.On the 42 nd day,the blood routine of each group was detected and the organ index was calculated.The levels of liver,kidney function and sex hormones in rats were detected by enzyme-linked immunosorbent assay.The histopathological changes of liver,kidney,ovary and testis were observed by hematoxylin-eosin(HE)staining.Results The testicular indexes of the normal group,the model group and the experimental group were(0.81±0.05)％,(0.97±0.06)％and(0.81±0.12)％;the estradiol contents were(63.90±16.19),(55.42±7.23)and(40.04±5.97)pg·mL-1;the testosterone contents were(1 290.96±257.94),(1 198.43±190.77)and(912.62±61.72)pg·mL-1,respectively.There were statistically significant differences in the above indexes between the model group and the experimental group(P＜0.01,P＜0.05).HE pathological results showed that Tripterygium glycosides tablets could cause abnormal histopathological changes of ovary and testis in CIA model rats.Conclusion Continuous administration of 8-fold clinical equivalent dose of Tripterygium glycosides tablets for 6 weeks can cause damage to the reproductive system of CIA rats.
关键词
雷公藤多苷片/Ⅱ型胶原诱导性关节炎模型/肝肾毒性/血液系统毒性/生殖毒性Key words
Tripterygium glycosides tablet/type Ⅱ collagen-induced arthritis model/hepatorenal toxicity/hematological toxicity/reproductive toxicity引用本文复制引用
基金项目
山西省基础研究计划(20210302123235)
山西中医药大学科技创新团队基金(2022TD1016)
出版年
2024
国家科技期刊平台
NSTL
万方数据