摘要
目的 研究二氢杨梅素(DMY)对力竭运动小鼠心肌氧化损伤的影响.方法 将C57BL/6小鼠分为对照组、模型组、阳性对照组和低、中、高剂量实验组,每组10只.对照组、模型组小鼠灌胃蒸馏水,低、中、高剂量实验组灌胃浓度为20、40、80 mg·kg-1二氢杨梅素,阳性对照组灌胃100 mg·kg-1 Vitamin C,每天1次,连续灌胃4周.给药结束后,用试剂盒法检测超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)水平,用蛋白质印迹法检测核因子E2相关因子2(Nrf2)、血红素加氧酶-1(HO-1)蛋白相对的表达水平.结果 对照组、模型组和低、中、高剂量实验组及阳性对照组的SOD分别为(57.81±6.92)、(26.85±2.74)、(33.68±4.52)、(39.74±3.95)、(48.97±4.26)和(39.22±3.54)U·mg-1,MDA 分别为(4.72±0.36)、(10.48±1.68)、(8.75±0.82)、(6.43±0.71)、(5.11±0.48)和(6.36±0.64)nmol·mg-1,LDH分别为(268.71±23.94)、(726.58±81.26)、(621.32±47.59)、(479.12±50.24)、(337.91±34.99)和(486.15±50.98)U·L-1,Nrf2 蛋白相对表达水平分别为 0.75±0.06、0.19±0.02、0.30±0.04、0.47±0.05、0.63±0.06和0.49±0.06,HO-1蛋白相对表达水平分别为0.83±0.08、0.27±0.05、0.39±0.04、0.52±0.03、0.77±0.07 和 0.55±0.06.对照组与模型组上述各指标比较,在统计学上差异均有统计学意义(均P＜0.05);模型组与阳性对照组和低、中、高剂量实验组上述各指标比较,在统计学上差异均有统计学意义(均P＜0.05).结论 二氢杨梅素可延缓力竭运动小鼠心肌氧化损伤,可能与Nrf2/HO-1通路有关.
Abstract
Objective To investigate the effect of dihydromyricetin(DMY)on myocardial oxidative damage in exhaustive exercise mice.Methods C57BL/6 mice were divided into control group,model group,positive control group and low,medium and high dose experimental groups and with 10 mice in each group.Mice in control group and model group were intragastricated with distilled water;20,40 and 80 mg·kg-1 dihydromyricetin were given by gavage in low,medium and high dose experimental groups,while mice in positive control group were intragastricated with 100 mg·kg-1 Vitamin C once a day for 4 weeks.After administration,superoxide dismutase(SOD),malondialdehyde(MDA)and lactate dehydrogenase(LDH)were detected by the kit.The expression of nuclear factor E2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)protein were detected by Western blot.Results SOD levels in control group,model group and low,medium,high dose experimental groups and positive control group were(57.81±6.92),(26.85±2.74),(33.68±4.52),(39.74±3.95),(48.97±4.26)and(39.22±3.54)U·mg-1;MDA were(4.72±0.36),(10.48±1.68),(8.75±0.82),(6.43±0.71),(5.11±0.48)and(6.36±0.64)nmol·mg-1;LDH were(268.71±23.94),(726.58±81.26),(621.32±47.59),(479.12±50.24),(337.91±34.99)and(486.15±50.98)U·L-1;Nrf2 protein expression were 0.75±0.06,0.19±0.02,0.30±0.04,0.47±0.05,0.63±0.06 and 0.49±0.06;the protein expression of HO-1 were 0.83±0.08,0.27±0.05,0.39±0.04,0.52±0.03,0.77±0.07 and 0.55±0.06,respectively.There were statistically significant differences between control group and model group(all P＜0.05);there were statistically significant differences in the above indexes between model group and positive control group,low dose experimental group,medium dose experimental group,high dose experimental group(all P＜0.05).Conclusion Dihydromyricetin can delay myocardial oxidative injury in exhaustive exercise mice,which may be related to Nrf2/HO-1 pathway.
基金项目
湖北省卫生健康委科研项目(2021-2022)(WJ2021M084)
国家科技期刊平台
NSTL
万方数据