Determination of four atypical antipsychotics in human urine by UPLC-MS/MS with polystyrene nanofibers as solid-phase extraction sorbent
Objective To develop a novel micro-extraction procedure based on polystyrene nanofibers as solid-phase extraction sorbent to extract four atypical antipsychotics including amisulpride,olanzapine,risperidone,paliperidone from human urine samples.Methods Polystyrene nanofibers were prepared by electrostatic spinning technique,and four atypical antipsychotics in human urine were extracted by a solid phase extraction column using polystyrene nanofibers as sorbent.The detection was performed on Waters XBridge BEH C18 with the mobile phase of0.1％formic acid aqueous solution(A)-acetonitrile(B)by a gradient elution at 0.25 mL·min-1 flow rate.The temperature of column was 40 ℃ and the injection volume was 3 μL.The method's selectivity,standard curves,quantification limit,precision,accuracy,extraction recovery,matrix effect,and stability were assessed.Results The linear ranges of the four analytes were found to be 1-100 ng·mL-1,with correlation coefficients all exceeding 0.996.The lower limit of quantification was 1 ng·mL-1,and the limit of quantitation ranged from 0.01-0.10 ng·mL-1.The intra-day and inter-day RSDs for the four analytes ranged from 2.34％-14.48％,with extraction recoveries all exceeding 75.75％.The matrix effect falls within the range of 85.00％-115.00％.The stability of samples placed in the autosampler for 24 hours exhibited RSDs ranging from 2.19％-12.06％,while the stability after three freeze-thaw cycles ranged from 3.65％-13.81％.Conclusion This method is sensitive and suitable for the determination of target analytes in complex matrices,and provides a reliable analytical tool for the detection of atypical antipsychotics.
polystyrene nanofiberssolid phase extractionultra performance liquid chromatography/tandem mass spectrometryantipsychotic drugsurine
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