Effects of isoliquiritigenin on exercise-induced fatigue and oxidative stress in mice
Objective To explore the effects of isoliquiritigenin on exercise-induced fatigue and oxidative stress in mice.Methods Exercise-induced fatigue model was established by swimming training methods.Fifty suaessfully molded mice were randomly divided into model group,positive control group,and experimental-L,-M,-H groups with 10 mice per group;another 10 normal mice were taken as normal.Normal group and model group were given 0.9％NaCl by intragastric administration.Experimental-L,-M,-H groups were given 10,20 and 40 mg·kg-1 isogliquiretin by intragastriction,respectively.Positive control group was given 200 mg·kg-1 American ginseng.The contents of malondialdehyde(MDA),the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were detected by the kit.The expression levels of peroxisome proliferator-activated receptor-γ-coactivator 1 α(PGC-1 α)and estrogen receptor-associated receptor α(ERRα)were detected by Western Blot.Results The contents of MDA in experimental-M,experimental-H,positive control,model,normal groups were(8.55±0.42),(4.76±0.33),(5.11±0.55),(13.45±1.32)and(2.78±0.46)nmol·mg-1;the SOD activities were(53.42±5.89),(65.37±6.19),(63.24±7.52),(33.43±4.57)and(79.52±8.95)U·mg-1;the GSH-Px activities were(72.89±6.99),(97.89±10.24),(99.74±11.65),(48.42±4.35)and(108.47±11.54)U·mg-1;the relative expression levels of PGC-1α protein were 0.52±0.07,0.75±0.06,0.73±0.10,0.23±0.04 and 0.83±0.08;the relative expression levels of ERRα protein were 0.46±0.04,0.62±0.07,0.65±0.09,0.19±0.04 and 0.77±0.05,respectively.The differences of above indexes were statistically significant between experimental-M,experimental-H,positive control groups with model group(all P＜0.05).Conclusion Isoglycyrrhizin can relieve exercise fatigue and oxidative stress in mice,and its mechanism is related to PGC-1 α and ERRα.
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