Effects of etomidate on the biological behavior of human retinoblastoma Y-79 cells by down-regulating the expression of circ_0000527
Objective To investigate the effect of etomidate(ETO)on the biological behavior of human retinoblastoma Y-79 cells and its possible mechanism.Methods Y-79 cells were cultured in vitro.They were divided into control group(conventional culture),experimental-L,-M,-H groups(treated with 0.2,0.4 and 0.8 μg·mL-1 ETO),si-NC group(transfected with si-NC),si-circ_0000527 group(transfected with si-circ_0000527),ETO+pcDNA group(transfected pcDNA after treated with 0.8 μg·mL-1 ETO)and ETO+pcDNA-circ_0000527 group(transfected pcDNA-circ_0000527 after treatment with 0.8 μg·mL-1 ETO).Cell proliferation was detected by methyl thiazolyl tetrazolium assay;cell clone formation was detected by plate cloning assay;scratch test and Transwell chamber were used to detect he ability of cell migration and invasion;the expression of circ_0000527 was detected by real-time fluorescence quantitative polymerase chain reaction;Western blot was used to detect the expression of N-cadherin and E-cadherin.Results The inhibition rates of cell proliferation in control group,experimental-L,-M,-H groups,si-NC group,si-circ_0000527 group,ETO+pcDNA group and ETO+pcDNA-circ_0000527 group were 0,(17.89±1.69)％,(38.43±3.10)％,(56.97±5.06)％,(5.78±0.56)％,(48.79±4.65)％,(58.40±4.81)％and(21.23±2.17)％;the number of clones formed were 87.15±6.11,70.91±6.26,52.32±5.19,38.68±3.42,88.73±6.81,44.18±4.83,36.26±3.62 and 72.14±5.69;scratch healing rates were(71.02±5.58)％,(54.08±4.97)％,(39.67±3.26)％,(23.98±2.77)％,(72.01±5.61)％,(30.16±2.26)％,(22.82±2.15)％and(60.17±5.83)％;the number of invasive cells were 119.80±12.93,88.78±6.78,70.73±6.18,52.78±4.29,120.18±12.48,62.34±5.88,50.88±4.07 and 92.06±8 60;the expression levels of circ_0000527 were 1.00±0.00,0.79±0.06,0.57±0.04,0.35±0.03,1.00±0.00,0.28±0.03,1.00±0.00 and 3.66±0.23,respectively.There were statistically significant differences in the above indexes between experimental-L,-M,-H groups and control group,between si-circ_0000527 group and si-NC group,between ETO+pcDNA-circ_0000527 group and ETO+pcDNA group(all P＜0.05).Conclusion ETO may inhibit Y-79 cell migration,invasion and proliferation by downregulating the expression of circ_0000527.
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