Effects of onion extract on the growth of fibroblasts in pathological scar through MAPK/ERK pathway
Objective To investigate the effect of onion extract on the growth of fibroblasts in pathological scar and its mechanism.Methods The isolated fibroblasts were randomly divided into blank group(normal cultured),experimental-L group(10 µmol·L-1 onion extract),experimental-M group(20 µmol·L-1 onion extract),experimental-H group(40 μmol·L-1 onion extract),anisomycin group[40 μmol·L-1 onion extract+10 µmoL·L-1 mitogen activated protein kinase(MAPK)signaling pathway activator anisomycin].Cell proliferation was detected by clonogenesis assay,cell apoptosis was detected by flow cytometry,cell migration was detected by wound healing assay,and relative expression levels of related proteins was detected by Western blot assay.Results The colonies number of blank group,experimental-L group,experimental-M group,experimental-H group and anisomycin group was 176.89±12.92,147.22±5.03,102.56±8.42,71.56±6.85 and 124.78±10.70,respectively;the apoptosis rates were(4.19±0.29)％,(8.67±0.75)％,(17.59±1.32)％,(25.75±2.10)％and(10.88±1.06)％,respectively;the migration rates were(0.60±0.05)％,(0.43±0.04)％,(0.31±0.02)％,(0.18±0.03)％and(0.51±0.04)％,respectively;the relative expression levels of phosphorylation-MAPK(p-MAPK)protein were 0.82±0.12,0.53±0.08,0.43±0.05,0.37±0.05 and 0.62±0.07,respectively;the relative expression levels of phosphorylated extracellular signal-regulated kinase 1/2(p-ERK1/2)protein were 0.93±0.10,0.73±0.07,0.60±0.07,0.47±0.07 and 0.76±0.07,respectively;the relative expression levels of alpha smooth muscle actin(α-SMA)protein were 0.80±0.09,0.60±0.04,0.33±0.03,0.21±0.02 and 0.77±0.08,respectively;the relative expression levels of collagen Ⅰ were 0.82±0.08,0.63±0.07,0.43±0.03,0.31±0.02 and 0.69±0.06,respectively.The above indexes in experimental-L,-M,-H groups were compared with those in blank group,and the differences were statistically significant(all P＜0.05);the above indexes in anisomycin group were significantly different from those in experimental-H group(all P＜0.05).Conclusion Onion extract may inhibit fibroblast growth,cell migration,fibrosis and extracellular matrix deposition by inhibiting MAPK/ERK pathway,induce cell apoptosis and alleviate the formation of pathological scar.
onion extractpathological scarfibroblastoverproliferationfibrosismitogen activated protein kinase/extracellular signal-regulated kinase pathway
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维普
