Effects of Maxing Shigan decoction on lung tissue damage in cough variant asthma rats
Objective To investigate the impact of Maxing Shigan decoction on lung tissue damage in cough variant asthma(CVA)rats by regulating the Notch1/Jagged1 signaling pathway.Methods The CVA rat model was established by ovalbumin+aluminum hydroxide sensitization method.After successful modeling,the rats were randomly divided into control group(conventional feeding),model group(4％ovalbumin 0.5 mL+2％aluminum hydroxide 0.2 mL),combined group(on the basis of the model group,16 g·kg-1 Maxing Shigan decoction and 200 mg·kg-1 Jagged1 were added respectively)and experimental-L,experimental-M,experimental-H groups(on the basis of the model group,4,8 and 16 g·kg-1 Maxing Shigan decoction were added respectively),with 15 rats in each group.The cough frequency and histopathological changes of the lung tissue were observed,and the expression level of Notch1 and Jagged1 protein were detected by Western blot.Results The coughing times of rats in control group,model group,experiment-L group,experiment-M group,experiment-H group and combined group were(2.04±0.08),(9.21±0.82),(7.38±0.60),(5.44±0.42),(3.14±0.32)and(7.88±0.65)times,respectively;the number of goblet cells in lung tissue was 13.65±1.24,59.66±5.54,47.21±4.12,34.58±3.01,23.54±2.06 and 39.25±3.68,respectively;the relative expression levels of Notch1 protein were 0.42±0.03,1.16±0.09,0.97±0.09,0.76±0.07,0.59±0.05 and 0.89±0.07,respectively;the relative expression levels of of Jagged1 protein were 0.36±0.03,1.09±0.04,0.92±0.09,0.70±0.07,0.46±0.04 and 0.79±0.07,respectively.The above indexes of the model group were compared with those of the control group,the experimental-L group,the experimental-M group and the experimental-H group,and the above indexes of the combined group were compared with those of the experimental-H group,and the differences were statistically significant(all P＜0.05).Conclusion Maxing Shigan decoction may improve lung tissue damage in CVA rats by inhibiting the activation of Notch1/Jagged1 signaling pathway.
万方数据
维普
