Correlation research of the polymorphism of HIF-1α gene rs11549465 and susceptibility to Helicobacter pylori in Tibetan gastric cancer patients living in Qinghai province
Objective To observe the relationship between the rs11549465 polymorphism of hypoxia-inducible factor 1α(HIF-1α)gene and susceptibility to Helicobacter pylori(Hp)infection in the Tibetan gastric cancer patients residing in Qinghai province.Methods The Tibetan gastric cancer patients residing in Qinghai province were divided into Hp infection and non-HP infection groups by cohort method.The rs1 1549465 polymorphism of the HIF-1α gene in venous blood,as well as the expression levels of HIF-1α,vascular endothelial growth factor(VEGF),P53 and Maspin in cancer tissue,were measured by polymerase chain reaction.Immunohistochemistry was used to detect the positive expressions of HIF-1α,VEGF,P53 and Maspin in cancer tissues.Results Hp infection and non-HP infection groups were enrolled 48 cases and 31 cases,respectively.The CC gene frequency at the HIF-1α rs11549465 sites in the Hp infection and non-HP infection groups were 58.33％(28 cases/48 cases)and 83.87％(26 cases/31 cases),with a statistically significant difference(P＜0.05).Logistic regression analysis showed that CT genotype at HIF-1α rs11549465 locus was the influencing factor of Hp infection in the the Tibetan gastric cancer patients residing in Qinghai province(odds ratio=5.026,95％confidence interval=1.810-13.951,P＜0.05).The positive expression rates of HIF-1α in Hp infection and non-HP infection groups were 89.58％and 64.52％;positive expression rates of VEGF were 91.67％and 70.97％;positive expression rates of P53 were 31.25％and 54.84％;positive expression rates of Maspin were 29.17％and 51.61％;the relative expression levels of HIF-1α mRNA were 2.48±0.41 and 2.23±0.39;the relative expression levels of VEGF mRNA were 2.42±0.38 and 2.14±0.31;the relative expression levels of P53 mRNA were 1.99±0.33 and 2.27±0.35;the relative expression levels of Maspin mRNA were 2.06±0.32 and 2.31±0.36,the differences were statistically significant(all P＜0.05).Conclusion The CT genotype at HIF-1α rs11549465 locus is a high-risk factor for Hp susceptibility the Tibetan gastric cancer patients residing in Qinghai province,and HIF-1 α can play a role in preventing gastric cancer by regulating the expression of VEGF,P53 and Maspin signal transduction pathways.
hypoxia inducible factor 1αrs11549465 gene polymorphismTibetan nationalitygastric cancer
万方数据
维普
