Objective To analyze the changes in pulmonary and intestinal microecology in elderly patients with chronic obstructive pulmonary disease(COPD)and coronary artery disease(CAD),as well as to investigate the immune regulatory mechanisms.Method 20 COPD patients,20 COPD patients with CAD,and 15 healthy elderly individuals were recruited from the Department of Geriatrics at a tertiary hospital in Sichuan Province between January 2022 and June 2022.Pulmonary and intestinal samples from the subjects were analyzed for microbial diversity,while cytokine levels in peripheral blood specimens were measured.Result There was no significant difference observed in the bacterial diversity index among the the groups.In the phylum level,compared with the control group,Firmicutes decreased while Proteobacteria and Actinobacteria increased within both pulmonary and intestinal microecologies of COPD-CAD group(P＜0.05).Compared with the COPD group,the intestinal microbiota of the COPD-CAD group were more Actinobacteria and less Proteobacteria(P＜0.05).In the genus level,the Pseudomonas increased while Streptococcus,Veillonella,Prevotella,Actinomyces decreased in the pulmonary microbiota of COPD-CAD group;the Escherichia increased while Bacteroides,Enterococcus,Lactobacillus decreased in the intestinal microbiota(P＜0.05).In terms of cytokine expression levels for COPD-CAD group,there was an increase observed for interleukin(IL)-2,IL-4,IL-6,and tumor necrosis factor(TNF)-a but a decrease for IL-10(P＜0.05).Conclusion Significant differences existed in both quantity and composition of lung and intestinal microbial flora among COPD patients with CAD,suggesting that microecology imbalances may contribute to systemic inflammatory responses increasing risk of COPD patients developing CAD.Modulating microbial community composition or its metabolites could be a potential therapeutic strategy for elderly individuals dealing with chronic comorbidities.
维普
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