Establishment of HPLC method for determination of salvianolic acid B,hydroxyl safflower yellow A,curcumin and oleanolic acid in Yuxuebi granules
AIM To establish an HPLC method for determining salvianolic acid B,hydroxyl safflower yellow A,curcumin,and oleanolic acid in Yuxuebi granules.METHODS Shimadzu CLC-ODS C18 chromatographic column separation was used.Acetonitrile(mobile phase A)-0.3％phosphoric acid solution(mobile phase B)was used as the mobile phase.Detection wavelengths were set at 286 nm for salvianolic acid B,403 nm for hydroxyl safflower yellow A,430 nm for curcumin,and 205 nm for oleanolic acid.The volume flow rate was set at 1.0 mL·min-1.The column temperature was set at 30℃,and the injection volume was 10 μL.RESULTS The mass concentration showed good linear relationships with the chromatographic peak area in the range of 0.096 6-9.660 0 μg·mL-1(salvianolic acid B),0.125 8-12.580 0μg·mL-1(hydroxyl safflower yellow A),0.098 1-9.810 0 μg·mL-1(curcumin)and 0.958 0-95.800 0 μg·mL-1(oleanolic acid).The average recoveries were 99.89％,100.39％,99.94％,100.01％,with RSD values of 0.60％,0.99％,1.01％,and 0.71％,respectively.CONCLUSION This method could apply to the quality control of Yuxuebi granule preparation.
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