Study of the regulatory mechanism of hepatic stellate cells by Mogroside based on TGF-β1/Smads signaling pathway
AIM To investigate the mechanism of anti-liver fibrosis of mogroside(Mog)via TGF-β1/Smads signaling pathway.METHODS Hepatic stellate cell line HSC-T6 was cultured in vitro.HSC-T6 cells were treated with serum containing various volume concentrations of Mog(5％,10％and 20％).The proliferation ability,toxicity release and migration ability of HSC-T6 were detected.The protein and mRNA expression levels of Smad2,3,4,7 and TGF-β1 in HSC-T6 were detected.The expression level of α-SMA in cells was detected.RESULTS Compared with the model group,Mog-containing serum at concentrations of 5％,10％and 20％significantly reduced the number of HSC-T6 proliferation within 24 h(P＜0.05),without any significant toxicity(P＞0.05).The cell migration rates in the 5％,10％,and 20％Mog groups were significantly lower than those in the model group(P＜0.05).All concentrations of Mog-containing serum were able to inhibit the expression of TGF-β1,Smad2,3,7 at both mRNA and protein levels in HSC-T6(P＜0.05).The protein and mRNA expression levels of Smad4 were higher than those of the model group(P＜0.05).The expression of α-SMA,a key marker of liver fibrosis,was also significantly down-regulated(P＜0.05).CONCLUSION Mog can inhibit the activation of HSC-T6 by regulating the expression of TGF-β1/Smads signaling pathway-related proteins and mRNA,thus playing an anti-hepatic fibrosis role.
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