基于TGF-β1/Smads信号通路探讨罗汉果甜苷抗肝纤维化的机制
Study of the regulatory mechanism of hepatic stellate cells by Mogroside based on TGF-β1/Smads signaling pathway
何晓璇 1肖刚 2罗陈亮 1吴桂有 1李悦 3康碧倩 3黄忠仕3
作者信息
- 1. 广西中医药大学药学院,南宁 530200
- 2. 右江民族医学院 临床医学院,百色 533000
- 3. 右江民族医学院 基础医学院,百色 533000
- 折叠
摘要
目的 基于TGF-β1/Smads信号通路探讨罗汉果甜苷(Mog)抗肝纤维化的机制.方法 体外培养大鼠肝星状细胞HSC-T6,以不同体积浓度的Mog含药血清对HSC-T6进行干预,检测HSC-T6的增殖能力、毒性释放情况和迁徙能力;检测细胞中TGF-β1 Smad2、3、4、7的蛋白和mRNA表达水平;检测细胞中α-SMA的表达水平.结果 在细胞增殖能力方面,5％、10％和20％Mog组的OD值低于模型组(P＜0.05).在毒性释放情况方面,2组差异无统计学意义(P＞0.05).5％、10％和20％Mog组的细胞迁徙率低于模型组(P＜0.05).5％、10％和20％Mog组的TGF-β1、Smad2、3、7的蛋白和mRNA表达水平均低于模型组(P＜0.05),Smad4的蛋白和mRNA的表达水平均高于模型组(P＜0.05),α-SMA的表达水平低于模型组.结论 Mog可通过调控TGF-β1/Smads信号通路相关蛋白和mRNA的表达,抑制HSC-T6的活化,进而起到抗肝纤维化作用.
Abstract
AIM To investigate the mechanism of anti-liver fibrosis of mogroside(Mog)via TGF-β1/Smads signaling pathway.METHODS Hepatic stellate cell line HSC-T6 was cultured in vitro.HSC-T6 cells were treated with serum containing various volume concentrations of Mog(5％,10％and 20％).The proliferation ability,toxicity release and migration ability of HSC-T6 were detected.The protein and mRNA expression levels of Smad2,3,4,7 and TGF-β1 in HSC-T6 were detected.The expression level of α-SMA in cells was detected.RESULTS Compared with the model group,Mog-containing serum at concentrations of 5％,10％and 20％significantly reduced the number of HSC-T6 proliferation within 24 h(P＜0.05),without any significant toxicity(P＞0.05).The cell migration rates in the 5％,10％,and 20％Mog groups were significantly lower than those in the model group(P＜0.05).All concentrations of Mog-containing serum were able to inhibit the expression of TGF-β1,Smad2,3,7 at both mRNA and protein levels in HSC-T6(P＜0.05).The protein and mRNA expression levels of Smad4 were higher than those of the model group(P＜0.05).The expression of α-SMA,a key marker of liver fibrosis,was also significantly down-regulated(P＜0.05).CONCLUSION Mog can inhibit the activation of HSC-T6 by regulating the expression of TGF-β1/Smads signaling pathway-related proteins and mRNA,thus playing an anti-hepatic fibrosis role.
关键词
罗汉果甜苷/肝纤维化/肝星状细胞/TGF-β1/Smads信号通路/含药血清Key words
mogroside/liver fibrosis/hepatic stellate cell/TGF-β1/Smads signaling pathway/drug-containing serum引用本文复制引用
基金项目
广西自然科学基金青年基金项目(2013GXNSFBA019197)
出版年
2024
国家科技期刊平台
NSTL
万方数据