Simultaneous determination of serum concentration of piperacillin/tazobactam in patients receiving continuous renal replacement therapy treatment by HPLC and its clinical application
AIM To establish a high-performance liquid chromatography(HPLC)method for simultaneous determination of piperacillin/tazobactam concentrations in the serum of patients receiving continuous renal replacement therapy(CRRT)treatment,so as to apply them to clinical practice for personalized drug design.METHODS The mobile phase was a mixture of acetonitrile and 0.1 mol·L-1 sodium dihydrogen phosphate solution.Acetonitrile was treated by gradient elution at a flow rate of 1 mL·min-1.The flow rate was 1 mL·min-1,the column temperature was 30℃,the detection wavelength was 220 nm,the pH value was 6.4,and the injection amount was 20 µL.The HPLC method was established to determine piperacillin/tazobactam concentration in patients receiving continuous renal replacement therapy treatment.The trough concentration of piperacillin/tazobactam in the serum of 12 sepsis patients with CRRT was determined by the method when the blood concentration reached a steady state.RESULTS There was a linear relationship between concentration range of 5-300 mg·L-1 and peak area for piperacillin(Y=0.134 6X-0.061 2,r=0.999 3).A linear relationship was obtained between concentration range of 1-50 mg·L-1and peak area for tazobactam(Y=0.060 7X+0.068 7,r=0.999 2).Average recoveries of piperacillin and tazobactam were 99.19％and 100.13％,respectively,with intra-day and inter-day precision less than 8％.The serum drug concentrations of piperacillin in 12 patients were all higher than the minimum inhibitory concentration(4 mg·L-1),but serum drug concentrations in 2 cases were below 4 times the minimum inhibitory concentration.The serum concentration of tazobactam was higher than 1 mg·L-1.CONCLUSION The HPLC method for detecting the serum concentration of piperacillin/tazobactam is simple to operate,with low instrument and detection costs,and can meet the needs of clinical practice testing.It is more suitable for adjusting personalized medication plans in primary medical institutions.
piperacillintazobactamhigh-performance liquid chromatographytherapeutic drug monitoring
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