RP-HPLC determination of caffeic acid,saikosaponin A,schaftoside and chlorogenic acid in Danshitong capsules
AIM To establish a reverse-phase high performance liquid chromatography(RP-HPLC)method for determination of caffeic acid,saikosaponin A,schaftoside and chlorogenic acid in Danshitong capsules.METHODS A RP-HPLC method was developed under the following conditions:A Waters spherisorb ODS2 C18 column(250 mm×4.6 mm,5μm)was employed with a flow rate of 1.0 mL·min-1 and a column temperature of 30℃.Gradient elution was carried out using acetonitrile(mobile phase A)and a 0.3%phosphoric acid solution(mobile phase B).Detection wavelengths for caffeic acid,saikosaponin A,schaftoside and chlorogenic acid were set at 323,210,272 and 327 nm,respectively.The injection volume was 10 μL for quantification of caffeic acid,saikosaponin A,schaftoside,and chlorogenic acid contents.RESULTS Caffeic acid,saikosaponin A,schaftoside and chlorogenic acid showed good linear relationships in concentration range of 0.029 91-0.299 1,0.474 0-4.740 0,0.071 18-0.711 8 and 0.385 2-3.852 0 μg·mL-1.Average recoveries were 100.18%,99.52%,100.20%and 100.23%,respectively.Both instrumental precision and method reproducibility were within 5.0%.CONCLUSION The method is characterized by its ease of operation,rapidity,and high efficiency,making it suitable for the determination of caffeic acid,saikosaponin A,schaftoside and chlorogenic acid content in the Danshitong capsule.
reverse-phase high performance liquid chromatographyDanshitong capsulecaffeic acidsaikosaponin Aschaftosidechlorogenic acid