首页|RP-HPLC法测定胆石通胶囊中咖啡酸、柴胡皂苷A、夏佛塔苷和绿原酸成分含量

RP-HPLC法测定胆石通胶囊中咖啡酸、柴胡皂苷A、夏佛塔苷和绿原酸成分含量

扫码查看
目的 建立一种测定胆石通胶囊中咖啡酸、柴胡皂苷A、夏佛塔苷和绿原酸含量的RP-HPLC法.方法 在色谱柱Waters spherisorb ODS2 C18(250 mm×4.6 mm,5 μm),柱流量 1.0 mL·min-1;柱温 30℃;以乙腈(流动相 A)-0.3%磷酸溶液(流动相B)为流动相进行梯度洗脱,咖啡酸、柴胡皂苷A、夏佛塔苷和绿原酸的检测波长分别为323、210、272和327 nm,进样量为10 μL的色谱条件下建立测定咖啡酸、柴胡皂苷A、夏佛塔苷和绿原酸成分含量的RP-HPLC法.结果 咖啡酸、柴胡皂苷A、夏佛塔苷和绿原酸分别在质量浓度 0.029 91~0.299 1、0.474 0~4.740 0、0.071 18~0.711 8和0.385 2~3.852 0 μg·mL-1 内具有良好的线性,平均回收率分别为100.18%、99.52%、100.20%和100.23%;仪器精密度和方法重复性良好(RSD均在5.0%以内).结论 该RP-HPLC法具有操作简便、快速和高效等优点,可用于胆石通胶囊中咖啡酸、柴胡皂苷A、夏佛塔苷和绿原酸含量的测定.
RP-HPLC determination of caffeic acid,saikosaponin A,schaftoside and chlorogenic acid in Danshitong capsules
AIM To establish a reverse-phase high performance liquid chromatography(RP-HPLC)method for determination of caffeic acid,saikosaponin A,schaftoside and chlorogenic acid in Danshitong capsules.METHODS A RP-HPLC method was developed under the following conditions:A Waters spherisorb ODS2 C18 column(250 mm×4.6 mm,5μm)was employed with a flow rate of 1.0 mL·min-1 and a column temperature of 30℃.Gradient elution was carried out using acetonitrile(mobile phase A)and a 0.3%phosphoric acid solution(mobile phase B).Detection wavelengths for caffeic acid,saikosaponin A,schaftoside and chlorogenic acid were set at 323,210,272 and 327 nm,respectively.The injection volume was 10 μL for quantification of caffeic acid,saikosaponin A,schaftoside,and chlorogenic acid contents.RESULTS Caffeic acid,saikosaponin A,schaftoside and chlorogenic acid showed good linear relationships in concentration range of 0.029 91-0.299 1,0.474 0-4.740 0,0.071 18-0.711 8 and 0.385 2-3.852 0 μg·mL-1.Average recoveries were 100.18%,99.52%,100.20%and 100.23%,respectively.Both instrumental precision and method reproducibility were within 5.0%.CONCLUSION The method is characterized by its ease of operation,rapidity,and high efficiency,making it suitable for the determination of caffeic acid,saikosaponin A,schaftoside and chlorogenic acid content in the Danshitong capsule.

reverse-phase high performance liquid chromatographyDanshitong capsulecaffeic acidsaikosaponin Aschaftosidechlorogenic acid

邱常萍、王永泉、孙霞

展开 >

山东省潍坊市中医院药学部,潍坊 261041

RP-HPLC 胆石通胶囊 咖啡酸 柴胡皂苷A 夏佛塔苷 绿原酸

2024

中国临床药学杂志
中国药学会

中国临床药学杂志

CSTPCD
影响因子:0.502
ISSN:1007-4406
年,卷(期):2024.33(6)