Effect of triple therapy on tuberculous pleurisy and its influence on serum SRSF6 and miR-506-3p levels
AIM To explore the efficacy of Wenpi Huoxue Decoction in the treatment of tuberculous pleurisy and the impact of serine/arginine-rich splicing factor 6(SRSF6)and microRNA-506-3p(miR-506-3p).METHODS A total of 98 patients with tuberculous pleurisy in the hospital from January 2021 to February 2023 were selected and randomly divided into control group(49 patients)and study group(49 patients).The control group was treated with pleural aspiration and anti-tuberculosis drugs,and the study group was treated with triple therapy(a combination of pleural aspiration,anti-tuberculosis drugs and Wenpi Huoxue decoction),and both groups were treated continuously for 2 months.The clinical efficacy and adverse events of 2 groups were compared.Traditional Chinese medicine(TCM)syndrome scores and pleural thickness before and after treatment in 2 groups were compared.Levels of inflammatory factors,adenosine deaminase(ADA),transforming growth factor-betal(TGF-β1),and SRSF6 in the pleural effusions of 2 groups before and after treatment were compared.The expression levels of miR-506-3p in the pleural effusions of 2 groups before and after treatment were compared.RESULTS The total effective rate of the study group(93.88％)was higher than that of the control group(79.59％),with a significant difference(P＜0.05).There was no significant difference in adverse reactions between 2 groups(P＞0.05).After treatment,the TCM syndrome score and pleural thickness of 2 groups were lower than before treatment,and the study group was lower than the control group(P＜0.05).After treatment,the level of SRSF6 in 2 groups was lower than that before treatment,and the level of miR-506-3p was higher than that before treatment,and the change range in the study group was larger than that in the control group(P＜0.05).After treatment,levels of interferon-induced protein 10(IP-10),interferon-y(IFN-γ),tumor necrosis factor-a(TNF-α),interleukin-17(IL-17),ADA and TGF-β,in 2 groups were lower than those before treatment,and those in the study group were lower than those in the control group(P＜0.05).CONCLUSION The auxiliary treatment with Wenpi Huoxue decoction can improve the clinical efficacy,alleviate clinical symptoms and reduce inflammatory responses in patients with tuberculous pleuritis.The mechanism underlying the effect might be associated with the ability of Wenpi Huoxue decoction to regulate levels of SRSF6 and miR-506-3p.
万方数据
维普
