Purification and Identify of Auricularia auricula Polysaccharide from Complex Enzyme Extraction
The protein was removed from crud auricularia auricula polysaccharide （AAP） by modified Sevag method. Two component AAP1 and AAP2 were gained by using Sephadex G-200 chromatography. The eluent was combined ,then dialysised and concentrated, freezed-drying. The pure AAP was gained. It was white powder. Then the pure AAP is identified by Sepharose A-200 chromatography, ultraviolet specrrum scanning. The result of Sepharose A-200 column chromatograpgy is that two polysaccharide components were single cleansing peak. The atlas of ultraviolet spectrum scanning indicates that there is not absorbing apex in 260nm and 280nm, and this indicates that protein, polypeptide and nucleic acid are not exist, and shows that the purification of AAP reaches the analyzing standard of physics and chemis- try. The result of IR chromatogran was that AAP1 had the characteristic absorb peak. , And the composition of monosaccharide of AAP was analyzed by chromatography, and the AAP1 is composed of glucose （Glu）, xylose（Xyl）, galactose（Gal）, mannose（Man）, arabinose（Arb）.
Auricularia auriculaPolysaccharideEnzyme i ExtractionPurification
国家科技期刊平台
NSTL
万方数据
维普
