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红豆越橘花色苷的分离及功能性研究

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采用活性跟踪的方法分离纯化野生红豆越橘提取物,并将有效组分进行抗癌细胞增殖活性测定。首先采用大孔吸附树脂X-5富集分离红豆越橘丙酮提取物,多酚含量和ABTS抗氧化做指标确定乙醇梯度洗脱有效组分,并命名为LB-1、LB-2、LB-3、LB-4,其总洗脱物命名为LB-MIX。用MTT法检测以上5个组分抗结肠癌HT29、卵巢癌Hela、肺癌A549和肝癌HepG-2细胞增殖活性,并选用环磷酰胺做阳性对照。结果显示,LB-MIX具有较好的抑制几株癌细胞的增殖活性。
Separation and Functionalities of Anthocyanins from Vaccinium vitis-idaea
In this experiment, purified extracts of Vaccinium vitis-idaea with the method of tracking activity, and determine the main anthocyanin composition by liquid chromatography-mass spectrometry techniques. First, enrichment and separation of acetone extracts of Vaccinium vitis-idaea by macroporous resin X-5, and determine the effective components of alcohol gradient by the phenolic contentation and scavenging activity of ABTS radical, and named LB-1, LB-2, LB-3, LB-4, the total eluate named LBMIX. The inhibition of HT29 colon cancer, Hela cervical cancer, A549 lung cancer, and HepG-2 liver cancer cell proliferation of five fractions were employed by MTT assay. The results showed that LB-MIX has a better inhibition of the four cancer cells proliferation activity.

Vaccinium vitis-idaea AnthocyaninsAntioxidant Antiproliferation

樊梓鸾、王振宇、左丽丽、田双起

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哈尔滨工业大学食品科学与工程学院,哈尔滨150090

哈尔滨工业大学食品科学与工程学院,哈尔滨150090/东北林业大学林学院,哈尔滨150040

红豆越橘 花色苷 抗氧化 抗细胞增殖

2011

中国林副特产
黑龙江省林副特产研究所

中国林副特产

影响因子:0.244
ISSN:1001-6902
年,卷(期):2011.(5)
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