Enzymolysis Technology and Antioxidant Activities of Bean Dregs Protein Peptides and Its Partial Properties
Adopting bean dregs protein as raw material and the scavenging rate of the hydrolysate to DPPH · as index,neutral protease has been screened out from the following four kinds of protease:trypsin,neutral protease,flavor protease and alkaline protease to obtain the optimal condition for enzymolysis by single factor and orthogonal experiment.Total reducing power and the scavenging effect of bean dregs protein peptides on hydroxyl radical,superoxide anion radical and their inhibition effects on both lipid peroxidation and partial properties have also been investigated with ascorbic acid (VC) as control sample.The results showed that the optimum condition of enzymolysis is pH value 7,temperature 70.0 ℃,concentration of bean dregs protein peptides 4 mg/mL and enzyme amount 100 U.Half inhibitory concentrations(IC50)value of VC and bean dregs protein peptides for scavenging hydroxyl radical and superoxide anion radical are 0.274 and 0.046 mg/mL,0.026 and 0.084 mg/mL respectively.Total reducing powers of VC and bean dregs protein peptides are all dosage-independent,expressing some inhibition capability on lipid peroxidation.Isoelectric point of bean dregs protein peptides is about 3.0 and the extraction rate increases gradually in the range of pH 3 ～ 12.
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万方数据
维普
