紫砂芸豆花色苷的超声/微波协同提取研究及其组成分析
Research on Ultrasonic/Microwave Synergistic Extraction of Anthocyanins from Purple Kidney Bean and Structure Identification
李良玉 1宋大巍 2刁静静 3贾鹏禹 3张丽萍4
作者信息
- 1. 黑龙江八一农垦大学国家杂粮工程技术研究中心,大庆163319;东北林业大学林学院,哈尔滨 150040
- 2. 黑龙江八一农垦大学食品学院,大庆163319
- 3. 黑龙江八一农垦大学国家杂粮工程技术研究中心,大庆163319
- 4. 黑龙江八一农垦大学国家杂粮工程技术研究中心,大庆163319;黑龙江八一农垦大学食品学院,大庆163319
- 折叠
摘要
以东北紫砂芸豆为原料,采用单因素试验和响应面优化试验研究超声/微波协同提取紫砂芸豆中花色苷的工艺参数,并采用液质连用色谱法对提取物进行不同浓度乙醇洗脱,进行组分分析.结果表明,超声/微波协同提取紫砂芸豆花色苷的有效工艺参数为:微波功率251 W,液料比13.7 mL/g,提取时间24.9min.不同浓度乙醇洗脱液的色谱分析发现,提取物中主要有5种花色苷物质,其中天竺葵素-3-葡萄糖苷、芍药素-3-葡萄糖苷含量较高,且主要集中在40％乙醇洗脱液中.本研究为大孔树脂纯化紫砂芸豆花色苷提供了参考,为紫砂芸豆花色苷的提取加工及工业应用提供依据.
Abstract
In this paper,purple kidney bean varieties from northeast of China was taken as the raw material,the ultrasonic/microwave synergistic extraction process of anthocyanins from purple bean were studied,and the structure was identified.As indicated in the results,the optimum ultrasonic/microwave synergistic extraction process parameters of anthocyanins from purple kidney bean were shown as following:microwave power 251 W,liquid to solid ratio 13.7 mL/g,extraction time 24.9 min.Chromatographic analysis of ethanol eluent with different concentration showed that there were mainly five kinds of anthocyanins substances,in which pelargonidin 3-glucoside,peony-3-glucoside were higher by of different concentration ethanol elution liquid quality analysis,pelargonidin3-glucoside,peony prime3-glucoside mainly concentrated in 40％ ethanol eluent.The research may provide reference for anthocyanin purification with macroporous resin,and as theory base for anthocyanin process and its industrial application.
关键词
紫砂芸豆/花色苷/超声/微波协同提取/组成分析Key words
purple kidney bean/anthocyanins/ultrasonic/microwave synergistic extraction/structure identification引用本文复制引用
基金项目
国家科技支撑计划(2011FU125X07)
出版年
2017
NETL
NSTL
维普
万方数据