Protective Effect of DHA Monoacylglyceride Algae Oil on Acute Alcohol-Induced Liver Injury in Mice
In this paper,the protective effect and mechanism of DHA monoglyceride(DHA-MAG)on acute alcoholic liver injury in mice were studied.The male mice used in experiments were randomly divided into five groups of normal group,model group,experimental group(DHA-MAG)and two positive control groups(triacylglycerols group(DHA-TAG);ethyl esters group(DHA-EE).After drug administration for 15 days,the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),triacylglyceride(TG),total cholesterol(TC),high-density lipoprotein(HDL),low-density lipoprotein(LDL)in mice serum were de-termined;meantime,the expression levels of toll-like receptor 4(TLR4),myeloid differentiation factor(MyD88),nuclear transcription factor-κB(NF-κB)of mice were examined.In addition,the histopathological changes of liv-er in mice were observed,and the intestinal bacteria diversities of feces were deeply analyzed.The obtained results indicated that the treatment by DHA-MAG significantly alleviated the acute alcohol-induced increases in serum ALT,AST,and ALP activities and reduced the levels of serum TG,TC and LDL in treated mice,while the level of serum HDL was increased.It was interesting to find that the trends of acute hepatic injury induced by alcohol were reduced after DHA-MAG treatment.Moreover,mice treated with DHA-MAG had a better protective effect on the liver injury induced by ethanol than those of treated with DHA-TAG and DHA-EE.Furthermore,DHA-MAG could significantly down-regulate the expression levels of TLR4,MyD88 and NF-Kb genes,and mitigate dysbiosis of intestinal flora induced by alcohol.Based on these results,it was concluded that DHA-MAG had protective effects on mice liver injury induced with alcohol by inhibiting TLR4/NF-κB pathway and maintaining the stability of intestinal flora.
DHA monoacylglyceridealcoholic liver injuryTLR4/NF-κB pathwayintestinal bacteriahepato-protective effects
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维普
