Effect of Yeast-Derived Feed Additive Aqueous Solution on Promoting Growth and Repairing Hydrogen Peroxide Damage in Grass Carp Primary Hepatocytes
In this experiment,three kinds of yeast-derived feed additive aqueous solutions were used as the test materials,aimming to investigate the promoting effect of yeast-derived feed additives on the growth of grass carp pri-mary hepatocytes and the alleviating effect of hydrogen peroxide(H2O2)on the oxidative damage of grass carp prima-ry hepatocytes.Grass carp primary hepatocytes were obtained by the enzymatic digestion method and plated at 200 µmol/L H2O2 for 1 h to establish an oxidative stress model.The cells were treated with yeast-derived feed additive aqueous solution at concentrations of 25,50,100,200,and 400 mg/L,to compare the growth promoting effects of three yeast-derived feed additive aqueous solutions on primary hepatocytes.The model cells were treated using the optimal growth promoting concentration of three yeast-derived feed additives,respectively,and the alleviating effect of yeast-derived feed additives on H2O2 induced oxidative damage was analyzed.The results indicated that three yeast-derived feed additive aqueous solutions could promote the proliferation of primary hepatocytes,and the optimal growth promoting concentrations of yeast culture YC-A,YC-B and yeast cell wall polysaccharide YCP were 100,200 and 100 mg/L,respectively,at which cell viability was significantly increased compared with the control group(P＜0.001).Compared to the H2O2 group,the three yeast-derived feed additive groups exhibited highly signifi-cant improvements in cell viability and MMP(P＜0.001),highly significant reductions in LDH activity in the cul-ture medium(P＜0.001),and highly significant reductions in the cell apoptosis rate and intracellular ROS levels(P＜0.01).Transmission electron microscopy revealed swollen,distorted,or absent mitochondria and numerous vacuoles in the cells of the H2O2 group,whereas improved mitochondrial swelling was observed in the three yeast-derived feed additive aqueous solution-treated groups.The transcriptome results suggested that YCP may protect cells from oxidative damage by increasing intracellular phase Ⅰ and Ⅱ metabolizing enzymes,whereas the protection of cells by YC may be achieved through the regulation of intracellular iron homeostasis.In conclusion,yeast-derived feed additive aqueous solution can promote the growth of primary hepatocytes in grass carp and have a certain repara-tive effect on oxidative damage in primary hepatocytes.
万方数据
维普
