摘要
以糙米为原料,建立利用低浓度氢氧化钠从稻米胚乳中分离蛋白体的方法.以蛋白体体积分数为指标,得到蛋白体分离条件为:碱液浓度14 mmol/L、液料比12 mL/g、搅拌时间90 min、超声功率90 W、超声温度35℃和超声时间40 min,蛋白体体积分数达20.10％.再经0.1、5.0 μm滤膜过滤及4.0 mol/L氯化钙去除残余淀粉,获得纯化的蛋白体.所得蛋白体呈微球形,有荧光;具有16、22、33、57 ku的蛋白亚基;X-衍射19.9°处显示β-折叠峰;红外1 464、1 534 cm-1属于Amide Ⅱ带;拉曼1 303、1 651 cm-1属于AmideⅢ和Amide Ⅰ带,均为蛋白质的特征吸收.因此,以低浓度氢氧化钠分离并经微孔滤膜和氯化钙纯化从稻米中获取蛋白体是可行的,这为研究稻米胚乳中蛋白体的结构和性质提供了方法学参考.
Abstract
Brown rice was used as the raw material,a method of separating protein bodies from rice endosperm with low concentration of sodium hydroxide was established.With the volume fraction of protein body as the index,the separation conditions of protein body were obtained as follows:alkali concentration of 14 mmol/L,liquid-to-material ratio of 12 mL/g,stirring time of 90 min,ultrasonic power of 90 W,ultrasonic temperature of 35 ℃ and ul-trasonic time of 40 min,and the volume fraction of protein body reached 20.10％.After another 0.1 μm and 5.0μm membrane filtration and 4.0 mol/L calcium chloride were used to remove residual starch to obtain purified protein bodies.The obtained protein body was microsphere with fluorescence.It had protein subunits of 16,22,33,57 ku;X-ray diffraction at 19.9° showed β-folding peak;infrared peaked at 1 464 cm-1 and 1 534 cm-1,being AmideⅡ band;Raman 1 303 cm-1 and 1 651 cm-1 were Amide Ⅲ and Amide Ⅰ bands,being the characteristic absorp-tion of proteins.Therefore,it was feasible to obtain protein bodies from rice by low concentration sodium hydroxide separation and purification through microporous filtration membrane and calcium chloride,providing a methodological references for studying the structure and properties of protein bodies in rice endosperm.
NETL
NSTL
万方数据