Isolation,Purification and Characterization of Protein Bodies from Rice
Brown rice was used as the raw material,a method of separating protein bodies from rice endosperm with low concentration of sodium hydroxide was established.With the volume fraction of protein body as the index,the separation conditions of protein body were obtained as follows:alkali concentration of 14 mmol/L,liquid-to-material ratio of 12 mL/g,stirring time of 90 min,ultrasonic power of 90 W,ultrasonic temperature of 35 ℃ and ul-trasonic time of 40 min,and the volume fraction of protein body reached 20.10％.After another 0.1 μm and 5.0μm membrane filtration and 4.0 mol/L calcium chloride were used to remove residual starch to obtain purified protein bodies.The obtained protein body was microsphere with fluorescence.It had protein subunits of 16,22,33,57 ku;X-ray diffraction at 19.9° showed β-folding peak;infrared peaked at 1 464 cm-1 and 1 534 cm-1,being AmideⅡ band;Raman 1 303 cm-1 and 1 651 cm-1 were Amide Ⅲ and Amide Ⅰ bands,being the characteristic absorp-tion of proteins.Therefore,it was feasible to obtain protein bodies from rice by low concentration sodium hydroxide separation and purification through microporous filtration membrane and calcium chloride,providing a methodological references for studying the structure and properties of protein bodies in rice endosperm.
riceprotein bodyisolation and purificationmorphologystructureidentification
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