To investigate the in vivo antioxidant effect of British red kidney bean antioxidant peptide components(BRKBPAPC),an oxidative stress zebrafish model was established using 2,2'-azobis(isobutyl)amidine dihydro-chloride induction to investigate the effect of BRKBPAPC on oxidatively stressed zebrafish.The results indicated that BRKBPAPC significantly increased(P＜0.05)the activity of catalase(CAT),superoxide dismutase(SOD),gluta-thione peroxidase(GSH-Px)and total antioxidant capacity(T-AOC),and significantly decreased(P＜0.05)the malondialdehyde(MDA)content in zebrafish,BRKBPAPC also had the ability to regulate zebrafish lipid metabo-lism,with the increase of treatment concentration zebrafish lipid metabolism index gradually restored to normal level;high dose of BRKBPAPC could make zebrafish behavior trajectory increased,the number of high-speed movement,time,distance significantly increased.At 21 d of high-dose antioxidant peptide treatment,CAT,SOD,T-AOC,and GSH-Px increased to(28.29±3.29),(126.59±6.39),(2.07±0.18),(84.75±7.77)U/mgprot,MDA content decreased to(3.35±0.29)nmol/mg prot,showing that BRKBPAPC had good in vivo antioxidant effect and lipid metabolism regulation ability.
British red kidney beanantioxidant peptideoxidative stresslipid metabolismzebrafishbehavior
NETL
NSTL
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