Fatty acids are widely existed in animal fats,plant oils and flavored foods,and some unsaturated fatty acids exhibit good hypolipidemic and antioxidant activities,being research hotspot in nutritional food and pharmaceu-tical industries.Since most fatty acids exist in the form of glycidyl esters,extraction and separation of free fatty acids from in natural foods is necessary.However,the lower purity of fatty acids obtained by traditional separation methods cannot satisfy the consumption demands of high purity fatty acids.High-speed counter current chromatography(HSCCC)is a liquid-liquid chromatographic technique without solid support,having the advantages of non-irre-versible adsorption and large single injection volume and has been widely used for the separation and purification of natural products in recent years.In this paper,by summarizing the relevant research works at home and abroad in re-cent years,the pros and cons of HSCCC compared with the traditional separation methods of fatty acids were dis-cussed,the solvent system,elution method,common detectors and their practical applications for the separation of fatty acids by high-speed countercurrent chromatography were reviewed,application potential was prospected,provi-ding references for the application of high-speed countercurrent chromatography in the separation and purification of fatty acids.
high-speed countercurrent chromatographyfatty acidsseparation and purificationapplication
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