Safety Evaluation of Fusarium Toxin in GM Rice and Analysis of Endophytic Fungus
The aim of this research was to evaluate the safety of fusarium toxins from shelf-period Genetically Modified(GM)rice,analyze the diversity of endophytic fungi in rice,and explore the possible causes of fusarium toxins from GM rice.Transgenic rice lines and their control parent varieties were collected to detect the toxin content of rice and its endophytic Fusarium strain by ELISA.The endophytic fungal communities of rice were detected by ITS high-throughput sequencing technique.Low doses of fusarium toxins were detected in all 4 rice samples,and the contents of ZEN,DON and T-2 ranged from 0.4 pg/kg to 25.8 µg/kg,and there were no significant differences between GM rice and parents(P＞0.05).Only 10.0 μg/kg FB1 was detected in GM rice sample FT16.All 16 en-dophytic sickle strains could produce ZEN,and 9 of them could also produce T-2 toxin.A total of 9 endophytic fun-gi phyla and 68 fungal genera were detected in 4 rice cultivars,in which the dominant fungi were Ascomycota,Fusar-ium and Curvularia.There was no significant difference in endophytic fungal α diversity index between the two GM rice samples and their parents(P＞0.05).The number of endophytic fungal marker species in GM rice was signifii-cantly lower than that in their respective parents.The content of fusarium toxin detected in rice samples from shelf period was much lower than the national standard,not posing food safety risk.The diversity index of endophytic fungi in GM rice was essentially the same as that of its parents,indicating that the variation of the genus was within the safe range.The prevalence of low doses of fusarium toxin and the presence of endophytic Fusarium fungi were more worthy of caution.
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