Optimization of Process Conditions for Xanthine Oxidase Activity Inhibitory Peptide of Rice Protein by Ultrasound-Assisted Protease Enzymolysis
Xanthine oxidase(XOD)inhibitory peptide was prepared by hydrolysis of rice protein with alkaline protease and its functional activity was evaluated in vitro.The response surface experiment was conducted to optimize the enzymolysis conditions with xanthine oxidase inhibition rate and hydrolysis degree as indexes,including protease type,amount of enzyme,substrate mass fraction,enzymolysis pH,enzymolysis temperature and enzymolysis time.on this basis,the influence of different ultrasonic treatments on enzymolysis process was discussed.the optimization re-sults were as follows:alkaline protease was used,substrate mass fraction was 5％,enzymatic hydrolysis temperature was 56 ℃,enzyme dosage was 8 000 U/g,enzymatic hydrolysis pH was 10,ultrasonic power was 70 W,and ultra-sonic enzymatic hydrolysis was carried out for 60 min.At these conditions,the IC50 of XOD inhibitory activity was 1.55 mg/mL and the IC50 values of ABTS+radical scavenging,hydroxyl radical scavenging and DPPH radical scav-enging were 0.49,12.48 mg/mL and 3.88 mg/mL,respectively.
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