首页|4种多酚对丙二醛诱导花生球蛋白氧化的改善作用

4种多酚对丙二醛诱导花生球蛋白氧化的改善作用

Ameliorating Effect of 4 Polyphenols Against Malondialdehyde-Induced Oxidation of Arachin

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为探究多酚对蛋白质氧化的改善作用,通过丙二醛建立花生球蛋白氧化体系,并采用槲皮素、儿茶素、绿原酸及没食子酸4种多酚干预氧化进程,分析其对花生球蛋白氧化程度和加工特性的影响.结果表明:4种多酚均对丙二醛氧化花生球蛋白有明显的改善作用,其中儿茶素使花生球蛋白的羰基浓度减少了98.10%,溶解度增加7.30%;没食子酸使花生球蛋白的疏水性降低35.57%;而槲皮素使花生球蛋白的游离氨基浓度增加了 83.31%;4种多酚均使内源荧光强度不同程度增大,其中没食子酸的干预最明显,最大增幅为38.37%.相关性分析进一步说明多酚干预可在一定程度上降低花生球蛋白的氧化进程.因此,4种多酚均可改善丙二醛对花生球蛋白氧化作用,其中以儿茶素的改善效果最明显.
The role of polyphenols in reducing protein oxidation was investigated in this study.A malondialde-hyde-induced oxidation arachin system was established,and the effects of quercetin,catechin,chlorogenic acid,and gallic acid on the oxidation degree and processing characteristics of arachin were examined.The results demon-strated that all four polyphenols significantly reduced the oxidation of arachin induced by malondialdehyde.Compared to the other polyphenols,catechin exhibited the highest reduction in carbonyl concentration at 98.10%,and solubili-ty increased by 7.30%.Gallic acid displayed the highest reduction in hydrophobicity relative to the other polyphenols at 35.57%,while quercetin revealed an 83.31%increase in free amino concentration.Moreover,all four polyphe-nols enhanced the endogenous fluorescence intensity to varying extents,with gallic acid demonstrating the most sub-stantial enhancement with a maximum increase of 38.37%.The correlation analysis further indicated that polyphenols could interfere with the oxidation process of arachin.In conclusion,all four polyphenols have potential in reducing malondialdehyde-induced oxidation in arachin,with catechin exhibiting the most significant effect.

arachinmalondialdehydeoxidationpolyphenolsameliorative effect

蒋丙婷、郭言、尹可宏、杨曦、孙健、张雪春

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西南林业大学云南省森林灾害预警与控制重点实验室,昆明 650224

西南林业大学生物与食品工程学院,昆明 650224

花生球蛋白 丙二醛 氧化 多酚 改善作用

国家自然科学基金云南省科技(农业联合)计划

317604402017FG0019-020

2024

中国粮油学报
中国粮油学会

中国粮油学报

CSTPCD北大核心
影响因子:1.056
ISSN:1003-0174
年,卷(期):2024.39(6)