7种富油微藻的超临界提取及脂肪酸分布特征
Supercritical Extraction of Lipid from 7 Microalgae Species and Fatty Acid Distribution Characteristics
林锡煌 1尹希杰 1袁秋兰 2张锐明 3项小燕3
作者信息
- 1. 自然资源部国家第三海洋研究所分析测试中心,厦门 361005
- 2. 龙岩学院化学与材料工程学院,龙岩 364000
- 3. 龙岩学院化学与材料工程学院,龙岩 364000;固体废弃物资源化利用福建省高校工程研究中心,龙岩 364000
- 折叠
摘要
富油微藻是制备生物燃料和提取高附加值不饱和脂肪酸的理想原料.为筛选具有高产油潜能的微藻,开发经济高效的微藻油脂提取工艺和检测方法,研究对7种富油微藻进行超临界CO2提取,并结合三氟化硼-甲醇衍生化、GC-MS分析了各微藻提取物中的35种脂肪酸组成和含量.结果表明,添加乙醇夹带剂后,各藻类的油脂收率由54.60％～82.16％提高至75.61％～104.46％.微藻中共检出18种脂肪酸,C16～C18系列脂肪酸质量分数均在55.97％以上,其中小球藻、三角褐指藻和青岛大扁藻的单不饱和脂肪酸质量分数分别为36.25％、31.81％和26.85％,是生物柴油理想的替代品.牟氏角毛藻的不饱和脂肪酸UFAs质量分数达到91.15％,可作为绿色医疗保健品的重要来源.
Abstract
Microalgae is an ideal raw material for biofuels and green healthy products.To screen the potential biodiesel microalgae and develop a cost-effective lipid extraction and analysis method,7 species of microalgae lipid were extracted using supercritical CO2 with ethanol as an entrainer.The composition of 35 fatty acids in microalgae extract were analyzed by boron trifluoride-methanol derivatization and GC-MS analysis.The results indicated that the oil recovery rates of algae increased from 54.60％-82.16％to 75.61％-104.46％after ethanol entrainer is added.A total of 18 types of fatty acids were detected in microalgae samples,mainly were C16 and C18 fatty acids(above 55.97％).Wherein,the content of MUFAs in Chlorella,Phaeodactylum tricornutum,and Platymonas sp.was 36.25％,31.81％and 26.85％,respectively,being ideal substitutes for biodiesel.The content of UFAs in Chaetoceros mueller reached 91.15％,being an important resource of green medical and health products.
关键词
富油微藻/超临界CO2/夹带剂/脂肪酸/分布特征Key words
microalgae/supercritical CO2 extraction/entrainer/fatty acid/distribution characteristics引用本文复制引用
基金项目
福建省教育中青年科技项目(JAT220368)
龙岩学院博士启动基金(LB2023007)
自然资源部第三海洋研究所基本科研业务费项目(海三科2009055)
出版年
2024
NETL
NSTL
万方数据