The lipid composition of Semen ziziphi spinosae oil was analyzed based on lipidomics in this study,and the functional characteristics were further analyzed.There were 384 lipid components in 19 subclasses and 5 catego-ries from Semen ziziphi spinosae oil.Its lipid molecules were medium and long chain lipids with carbon chain lengths of 35~54,accounting for 66.60%,and unsaturated lipids accounted for 95.70%.Compared with the vegetable oil reported at present,there were 292 subtypes of glycerolipids(GL)in Semen ziziphi spinosae oil,being the most reported vegetable oil at present,and the content of diglycerides(DG)reached 1 090 607.62 mol/g.The prelimina-ry functional analysis indicated that the hypolipidemic activity of Semen ziziphi spinosae oil may be related to its large amount of DGs.