摘要
本实验旨在研究月桂酸(lauricacid,LA)对肉鸡肌肉品质、常规营养成分和抗氧化性能的调控作用.实验选用480只1日龄AA肉仔鸡,随机分为4组,每组8个重复,每个重复15只鸡,分别饲喂基础饲粮(对照组)和基础饲粮分别添加75 mg/kg金霉素(抗生素组)、500 mg/kg月桂酸(月桂酸低剂量组;LA500)、1000 mg/kg月桂酸(月桂酸高剂量组;LA1 000)的实验饲粮,实验期为42 d.结果表明:与对照组相比,饲粮添加500 mg/kg月桂酸提高了肉鸡腿肌24 h的pH、黄度(b*)值和粗脂肪含量,增加了过氧化氢酶(CAT)的活力(P＜0.05),上调了 SOD2、CAT、GPX1、Nrf2、HO-1的基因表达量(P＜0.05),降低了 24 h和48 h的滴水损失(P＜0.05),饲粮添加1 000 mg/kg月桂酸提高了肉鸡腿肌24 h的pH、红度(a*)值、黄度(b*)值、粗脂肪含量(P＜0.05),增加了总抗氧化能力(T-AOC)、总超氧化物歧化酶(SOD)、CAT的活力(P＜0.05),上调了SOD1、SOD2、CAT、GPX1、Nrf2、HO-1的基因表达量(P＜0.05),降低了 24 h的亮度(L*)值、滴水损失、48 h的滴水损失和丙二醛(MDA)的含量(P＜0.05);与抗生素组相比,添加500 mg/kg月桂酸的饲粮提高了肉鸡腿肌CAT的含量,降低了肉鸡腿肌24 h的滴水损失、MDA的含量(P＜0.05),饲粮添加1 000 mg/kg月桂酸提高了肉鸡腿肌CAT的含量,降低了肉鸡腿肌24、48 h的滴水损失和MDA的含量(P＜0.05).月桂酸能够改善肉鸡肌肉品质和抗氧化能力,激活 Nrf2 信号通路相关基因的表达,且效果优于抗生素,饲粮添加1 000 mg/kg月桂酸更佳.
Abstract
In this experiment,the regulatory effects of lauric acid(LA)on muscle quality,conventional nutri-ents and antioxidant properties of broilers were investigated.A total of 480 1-day-old AA broilers were randomly divided into 4 groups with 8 replicates per group and 15 broilers per replicate.Broilers were fed a basal diet(control group),and the basal diet supplemented with 75 mg/kg aureomycin(antibiotic group),500 mg/kg lauric acid(low-dose lauric acid group,LA500),and 1 000 mg/kg lauric acid(lauric acid high-dose group,LA1 000),respectively.The experiment lasted for 42 days.The results indicated that,compared with the control group,the yellow(b*)value,pH at 24 h and ether extract content in leg muscle was increased by dietary supplementation of 500 mg/kg lauric acid,the level of catalase(CAT)was increased,the expression levels of SOD2,CAT,GPXl,Nrf2 and HO-1 genes were up-regulated,and drip loss at 24 h and 48 h of leg muscle in broilers was decreased(P＜0.05).PH,a*,and b*values at 24 h and the contents of ether extract in leg muscle were increased by dieta-ry 1 000 mg/kg lauric acid,the levels of total antioxidant capacity(T-AOC),total superoxide dismutase(SOD)and CAT were increased,the gene expression levels of SOD1,SOD2,CAT,GPX1,Nrf2 and HO-1 were up-regu-lated,the brightness(L*)value and drip loss at 24 h were decreased,drip loss at 48 h was decreased,and the con-tent of malondialdehyde(MDA)in leg muscle of broilers was reduced(P＜0.05).Compared with antibiotic group,the level of CAT was increased by dietary 500 mg/kg lauric acid,the drip loss at 24 h was decreased,and the con-tent of MDA of leg muscle in broilers was reduced(P＜0.05).The level of CAT was increased by dietary supple-mentation with 1 000 mg/kg lauric acid,the drip loss at 24 h and 48 h was decreased,and the content of MDA in leg muscle of broilers was reduced(P＜0.05).Lauric acid could improve muscle quality and antioxidant capacity,acti-vate the related genes expression of Nrf2 signaling pathway,and the effect was better than antibiotics;it was better to add 1 000 mg/kg lauric acid to the diet.
基金项目
浙江省领军型创新创业团队项目(2020R01015)
浙江农林大学国家级大学生创新创业训练计划(202210341048)
浙江省重点农业企业研究院项目(2021Y30004)
NETL
NSTL
万方数据