同位素稀释液相色谱-串联质谱法测定小麦中脱氧雪腐镰刀菌烯醇含量的不确定度评价
Uncertainty Evaluation of Deoxynivalenol Content in Wheat Determined by Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry
胡婷婷 1高晓芳 2弓浩然 2王争艳 3洪冰 2苏淼冉2
作者信息
- 1. 河南工业大学粮食和物资储备学院,郑州 450001;郑州市粮食科学研究所,郑州 450001
- 2. 郑州市粮食科学研究所,郑州 450001
- 3. 河南工业大学粮食和物资储备学院,郑州 450001
- 折叠
摘要
采用同位素稀释液相色谱-串联质谱法测定小麦中脱氧雪腐镰刀菌烯醇,对测定过程中可能引入的不确定度进行分析评定,为建立有效的质量控制方法提供参考.结果表明:小麦中脱氧雪腐镰刀菌烯醇含量为306 μg/kg时,其扩展不确定度为59μg/kg(k=2),影响检测结果不确定度的因素主要是标准溶液配制,其次是样品称量和样品基质,而样品提取及定容、标准曲线拟合及测量重复性等引入的不确定度较小.
Abstract
The possible uncertainties in the determination of deoxynivalenol in wheat by isotope dilution liquid chromatography-tandem mass spectrometry were analyzed and evaluated to provide reference for the establishment of effective quality control methods.The results indicated that when the content of deoxynivalenol in wheat was 306μg/kg,the expanded uncertainty was 59 μg/kg(k=2),the main factor affecting the uncertainty was the preparation of standard solution,followed by sample weighing and sample matrix,while the uncertainty introduced by sample ex-traction and constant volume,standard curve fitting and measurement repeatability was small.
关键词
不确定度/脱氧雪腐镰刀菌烯醇/高效液相色谱-串联质谱/同位素/小麦Key words
uncertainty/deoxynivalenol/HPLC-MS/MS/isotope/wheat引用本文复制引用
基金项目
河南省科技研发计划联合基金(应用攻关类)(2022)(222103810069)
出版年
2024
NETL
NSTL
万方数据