响应面法优化甜高粱籽粒总黄酮提取及主要组分测定
Optimization of Extraction Process by Response Surface Methodology and Determination of Main Components of Total Flavonoids from Sweet Sorghum Grain
王斐 1何振富 1王琪 2谢建鹏 1陈平 1李婷3
作者信息
- 1. 甘肃省农业科学院畜草与绿色农业研究所,兰州 730070
- 2. 兰州大学功能有机分子化学国家重点实验室,兰州 730013
- 3. 甘肃省农业科学院畜草与绿色农业研究所,兰州 730070;甘肃省农业科学院农业质量标准与检测技术研究所,兰州 730070
- 折叠
摘要
确定甜高粱籽粒总黄酮最佳提取工艺,测定籽粒总黄酮主要组分及其含量,并评价其体外抗氧化性.以陇甜粱2号成熟期籽粒为研究对象,以黄酮含量为指标,在单因素实验基础上,利用响应面优化法确定甜高粱籽粒总黄酮最佳提取工艺为料液比1.0∶15.5(g/mL)、乙醇体积分数70％、提取温度80 ℃、超声时间29.5 min,所得黄酮含量理论为34.14 mg/g,实际可达(34.22±0.30)mg/g.采用高效液相色谱法(HPLC)测得陇甜粱2号籽粒总黄酮中(+)-儿茶素和芦丁含量最高,分别为3.557 8、2.114 6 mg/g.籽粒总黄酮清除羟基自由基(·OH)和1,1-二苯基-2-三硝基苯肼(DPPH)自由基的IC50分别为0.544 2、0.143 8 mg/mL,1 mg/mL籽粒总黄酮溶液对DPPH自由基的清除率可达(93.31±0.60)％,具有良好的体外抗氧化性.
Abstract
In this paper,the optimal extraction process of total flavonoids of sweet sorghum grain was deter-mined,the main components and contents were measured,and the antioxidant activity in vitro was evaluated.Based on the single factor experiments,the optimal extraction process of total flavonoids from sweet sorghum(Longtianliang 2)grain at maturity was determined by response surface methodology with the content of flavonoids as the index,in-cluding the material-to-liquid ratio of 1.0∶15.5(g/mL),the ethanol concentration of 70％,the extraction tem-perature of 80 ℃,and the ultrasound duration of 29.5 minutes.The obtained flavonoid content was 34.14 mg/g in theory,and(34.22±0.30)mg/g in practice.The contents of catechin and rutin in the total flavonoids of Longtian-liang 2 grains were the highest,being 3.557 8 mg/g and 2.114 6 mg/g,respectively.The IC50 value of the extract for scavenging hydroxyl radical(·OH)and DPPH radical was 0.544 2 mg/mL and 0.143 8 mg/mL,respectively.The DPPH radical scavenging rate of 1 mg/mL total flavonoids solution was up to(93.31±0.60)％.The results indicated that it was feasible to extract total flavonoids from sweet sorghum grain by this process,and the extract had good antioxidant properties in vitro.
关键词
甜高粱/籽粒/总黄酮/高效液相色谱/抗氧化Key words
sweet sorghum/grain/total flavonoids/HPLC/antioxidant activity引用本文复制引用
基金项目
嘉峪关市科技计划(21-24)
甘肃省农科院农业科技创新专项(2021GAAS45)
兰州市科技计划(2022-2-96)
出版年
2024
NETL
NSTL
万方数据