中国粮油学报2024,Vol.39Issue(7) :102-109.DOI:10.20048/j.cnki.issn.1003-0174.000846

谷氨酰胺转氨酶对无麸质高水分组织蛋白品质的影响

Effects of Transglutaminase on the Quality Characteristics of Gluten-free High-moisture Textured Protein

安红周 杨嘉菲 朱雪庆 孟雪艳 郭益廷 李盘欣 罗琼
中国粮油学报2024,Vol.39Issue(7) :102-109.DOI:10.20048/j.cnki.issn.1003-0174.000846

谷氨酰胺转氨酶对无麸质高水分组织蛋白品质的影响

Effects of Transglutaminase on the Quality Characteristics of Gluten-free High-moisture Textured Protein

安红周 1杨嘉菲 1朱雪庆 1孟雪艳 1郭益廷 1李盘欣 2罗琼3
扫码查看

作者信息

  • 1. 河南工业大学粮油食品学院,郑州 450001;小麦和玉米深加工国家工程研究中心,郑州 450001
  • 2. 河南省南街村集团有限公司,临颍 462600
  • 3. 小麦和玉米深加工国家工程研究中心,郑州 450001
  • 折叠

摘要

为了提高无麸质高水分组织蛋白的品质特性,本研究将不同梯度含量的谷氨酰胺转氨酶加入大米蛋白与玉米醇溶蛋白混料中进行挤压实验以进行品质改善,并对组织蛋白进行理化特性的测定与分析.高水分组织蛋白中水分的主要存在形式是不易流动水,低场核磁共振结果表明,谷氨酰胺转氨酶的添加可以促进产品中的结合水转换为不易流动水;随着酶添加量增加,储能模量与损耗模量增大,蛋白结构更趋于稳定.组织蛋白的持水性能与持油性能得到改善,硬度及咀嚼性显著增高(P<0.05),组织化度呈现先增高后降低的趋势,且酶质量分数为0.5%时,产品组织化度达到最大值1.58.谷氨酰胺转氨酶可以显著影响高水分组织蛋白的品质,添加质量分数0.2%~0.5%的谷氨酰胺转氨酶能促进产品形成丰富的纤维结构,使产品具有更好的品质.

Abstract

In order to improve the quality characteristics of gluten-free high-moisture textured protein,in this study,transglutaminase was added with different gradient contents to the mixture of rice protein and Zein for extrusion experiments to improve the quality,and the physicochemical properties of tissue proteins were determined and ana-lyzed.The main form of water in high-moisture textured protein was not easy to flow,and the low-field NMR re-sults indicated that the addition of transglutaminase promoted the conversion of bound water in the product to water which was not easy to flow.With the increase of enzyme addition,the storage modulus and the loss modulus in-creased,and the protein structure became more stable.The water holding capacity and oil holding capacity of high-moisture textured protein were improved,the hardness and chewiness were significantly increased(P<0.05),the texture degree showed a trend of first increasing and then decreasing,and the degree of organization of the product reached the maximum value of 1.58 at 0.5%enzyme addition.In conclusion,transglutaminase significantly affected the quality of high-moisture textured protein,and the addition of0.2%-0.5%transglutaminase can promote the formation of rich fiber structure of the product,so that the product had better quality.

关键词

高水分组织蛋白/无麸质/谷氨酰胺转氨酶

Key words

high-moisture textured protein/gluten-free/transglutaminase

引用本文复制引用

基金项目

小麦和玉米深加工国家工程研究中心开放课题项目(NL2023009)

国家重点研发计划项目(2022YFD2101405)

河南工业大学博士基金项目(2021BS038)

出版年

2024
中国粮油学报
中国粮油学会

中国粮油学报

CSTPCD北大核心
影响因子:1.056
ISSN:1003-0174
段落导航相关论文