In this study,soybean oil was refined by enzymatic degumming and adsorption deacidification.The process was optimized by single factor experiment and orthogonal experiment to obtain suitable refining conditions of soybean oil.The results indicated that the optimum conditions for degumming by phospholipase C enzymatic method were pH of 5.4,enzyme dosage of 10.0 µL/kg and enzymatic hydrolysis temperature of 45.0 ℃.Under these condi-tions,the phosphorus of soybean crude oil was reduced to 7.3 mg/kg.The optimal deacidification conditions were holding time of 40.0 min,adding temperature of 90.0 ℃,water mass fraction of 3.0％and flocculating dose of 0.7％.Under these conditions,soybean oleic acid value was 0.42 mg KOH/g and phosphorus content was 6.2 mg/kg.After enzymatic degumming and adsorption deacidification,the phosphorus content and acid value of soybean crude oil were significantly reduced,and the quality was improved.
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