玉米赤霉烯酮降解酶热稳定性及底物特异性改造策略
Strategies for Thermostability and Substrate Specific Modification of Zearalenone Degrading Enzymes
秦立晔 1刘晓萌 2王寿南 2张晓琳 2印铁 2任顺成 3仉磊2
作者信息
- 1. 河南工业大学粮油食品学院,郑州 450001;中粮营养健康研究院;北京市畜产品质量安全源头控制工程技术研究中心,北京 102209
- 2. 中粮营养健康研究院;北京市畜产品质量安全源头控制工程技术研究中心,北京 102209;南京财经大学食品科学与工程学院;江苏省现代粮食流通与安全协同创新中心,南京 210023
- 3. 河南工业大学粮油食品学院,郑州 450001
- 折叠
摘要
玉米赤霉烯酮(zearalenone,ZEN)及其衍生物是污染范围较大的真菌毒素,广泛存在于玉米、大麦、小麦和高粱等谷物及其副产品中,对饲料行业和畜牧养殖业造成严重的经济损失.利用生物技术对ZEN及其衍生物进行降解和脱除是未来解决ZEN污染的重要途径.然而,目前发现的ZEN降解酶热稳定性差,催化效率低,难以满足工业生产中高温、酸、碱等极端条件的需求.本文综述了近年来利用蛋白质工程提升ZEN降解酶热稳定性和底物特异性的改造策略,阐明ZEN降解酶改造的研究进展及存在问题,为开发ZEN降解酶的工程改造提供参考.
Abstract
Zearalenone(Zen)and its derivatives are one of the mycotoxins with a wide range of contamination,and widely exist grains and their byproducts,(such as corn,barley,wheat,sorghum et al.),posing a serious threat to animal and human health.The degradation and removal of ZEN and its derivatives by biotechnology will be the ma-jor method to solve the pollution of ZEN in the future.However,it is currently known that ZEN degrading enzyme has poor thermal stability and low catalytic efficiency,and difficult to meet the requirements of industrial production.In this paper,the modification strategies with protein engineering for improving the thermal stability and substrate speci-ficity of ZEN degrading enzymes were reviewed,and the research advance and problems of the ZEN degrading en-zymes were elucidated to provide reference for the development of engineering modification of ZEN degrading enzymes.
关键词
玉米赤霉烯酮/降解酶/热稳定性/底物特异性/分子改造Key words
zearalenone/degrading enzymes/thermal stability/substrate specificity/molecular modification引用本文复制引用
基金项目
国家重点研发计划(十四五)(2021YFC2103003)
出版年
2024
NETL
NSTL
万方数据