A Method for Determination of Aflatoxin B,in Corn and Rice by Using Ag+/Cys Mediated Nucleic Acid Probe Cascade Immunofluorescence Assay
Based on the fluorescence intensity changes of Glucose Oxidase(GOx)catalyzed Ag+/Cys mediated G-rich ssDNA/Tb3+nucleic acid probe,a novel double cascade fluorescence signal amplification system was devel-oped.The fluorescence system had a high sensitivity response to Gox(as low as 0.05 μg/mlGOx).This system was combined with magnetic separation immune system to establish a high-sensitivity double cascade signal amplification fluorescence immunoassay method for detection AFB,in foods.The detection limit of this method for AFB1 was 0.05 ng/mL.The results of the addition recovery experiment indicated that the recovery rate of AFB1 was 85.09％～105.2％,with the coefficient of variation(CV)was 6.8％～15.7％.
NETL
NSTL
万方数据
