Ag+/Cys介导的核酸探针级联荧光信号免疫检测法测定玉米和大米中黄曲霉毒素B1
A Method for Determination of Aflatoxin B,in Corn and Rice by Using Ag+/Cys Mediated Nucleic Acid Probe Cascade Immunofluorescence Assay
李亚楠 1解立斌 1陈佳 1韩爱云 1张文娜 1剧慧栋 1冯佩 2张媛媛 1胡连霞1
作者信息
- 1. 石家庄学院化工学院,石家庄 050035;石家庄市食品工程技术创新中心,石家庄 050035
- 2. 石家庄市疾病预防控制中心理化检验科,石家庄 050011
- 折叠
摘要
以葡萄糖氧化酶(GOx)催化Ag+/Cys介导的富G-ssDNA/Tb3+核酸探针体系引发荧光强度变化为方法学基础,开发了一种新型ELISA双重级联荧光信号放大系统,对葡萄糖氧化酶具有高的灵敏度响应(低至0.05 μg/mLGOx).将此体系与对GOx负载的纳米微球为信号探针的磁分离免疫体系相结合,建立了一种用于食品中AFB,的高灵敏的双重级联信号放大的荧光免疫检测方法.该方法对AFB1的检出限为0.05 ng/mL,在加标的玉米和大米样品中,添加回收实验结果表明,AFB,的回收率在85.09％～105.2％之间,变异系数(CV)值为 6.8％～15.7％.
Abstract
Based on the fluorescence intensity changes of Glucose Oxidase(GOx)catalyzed Ag+/Cys mediated G-rich ssDNA/Tb3+nucleic acid probe,a novel double cascade fluorescence signal amplification system was devel-oped.The fluorescence system had a high sensitivity response to Gox(as low as 0.05 μg/mlGOx).This system was combined with magnetic separation immune system to establish a high-sensitivity double cascade signal amplification fluorescence immunoassay method for detection AFB,in foods.The detection limit of this method for AFB1 was 0.05 ng/mL.The results of the addition recovery experiment indicated that the recovery rate of AFB1 was 85.09％～105.2％,with the coefficient of variation(CV)was 6.8％～15.7％.
关键词
黄曲霉毒素B1(AFB1)/富G单链DNA/铽离子(Tb3+)/抗体/荧光免疫检测Key words
aflatoxin B1(AFB1)/G-rich ssDNA/terbium ion(Tb3+)/antibody/immunofluorescence assay引用本文复制引用
基金项目
石家庄市科学技术研究与发展计划(211170641)
石家庄学院博士科研启动基金(21BS002)
石家庄学院校级教学改革研究与实践项目(JGXM-202170G)
河北省高层次人才项目(B20221010)
出版年
2024
NETL
NSTL
万方数据