促乙醇脱氢酶活性的小麦肽制备及其醒酒功效评价
Preparation of Wheat Peptides with Promoting Ethanol Dehydrogenase Activity and Evaluation of Anti-alcohol Efficiency
方颂平 1冯盼盼 2刘露 1王小露 2刘飞翔 1徐桢 2王卓2
作者信息
- 1. 亳州学院,亳州 236800;养生型配制酒亳州市重点实验室,亳州 236800
- 2. 亳州学院,亳州 236800
- 折叠
摘要
为制备高醒酒活性的小麦肽,采用不同蛋白酶对小麦面筋蛋白进行酶解,以模拟胃肠道消化后对乙醇脱氢酶(alcohol dehydrogenase,ADH)激活率为指标进行酶解工艺研究.利用超滤和乙醇溶解分离技术,制备高促ADH活性的小麦肽,并通过动物实验进行醒酒功效评价.结果表明,胰蛋白酶酶解制备的小麦肽对ADH激活作用最显著,响应面优化得到最佳酶解条件为酶质量分数(E/S)0.5％、小麦面筋蛋白质量浓度7.0 mg/mL、酶解时间2.3 h;采用超滤分离获得分子质量为1～3 ku组分对ADH激活率最高,达到85.49％,进一步采用体积分数40％的乙醇溶解分离,其醇溶组分经模拟胃肠道消化处理后对ADH激活率达到120.3％;小鼠翻正反射及旷场精细行为实验表明,制备的小麦肽具有一定的醒酒功效,对小鼠酒后不适感,具有缓解作用.本研究可为小麦面筋蛋白醒酒肽的制备提供参考.
Abstract
To prepare wheat gluten peptides with high anti-alcohol activity,different proteases were used to en-zymatically hydrolyze wheat gluten,and the enzymolysis process was determined by the activation rate of alcohol de-hydrogenase(ADH)after simulated gastrointestinal digestion.The highly anti-alcohol active components were ob-tained by ultrafiltration and ethanol dissolution,and the sobering effect was verified by animal experiments.The re-sults indicated that the wheat peptides prepared by trypsin enzymolysis had the most significant effect on ADH activa-tion and the optimal enzymolysis conditions obtained by response surface optimization include 0.5％of enzyme dosage(E/S),7.0 mg/mL of wheat gluten concentration,and 2.3 h of enzymolysis time.The component with molecular weight of 1-3 ku obtained by ultrafiltration separation had the highest ADH activation rate of 85.49％,and the acti-vation rate to ADH of the alcohol-soluble component obtained from further separation by 40％ethanol dissolution reached 120.3％.The results of righting reflex and open-field fine behavior indicated that the wheat gluten peptide had a certain effect on sobering up and alleviating the discomfort of mice after drinking alcohol.This study can pro-vide a reference for the preparation of wheat gluten anti-alcohol peptides.
关键词
小麦肽/模拟胃肠道消化/乙醇脱氢酶/旷场精细行为Key words
wheat peptide/simulated gastrointestinal digestion/ethanol dehydrogenase/open-field fine behavior引用本文复制引用
基金项目
安徽省高等学校科研项目(KJ2020A0766)
出版年
2024
NETL
NSTL
万方数据