To prepare wheat gluten peptides with high anti-alcohol activity,different proteases were used to en-zymatically hydrolyze wheat gluten,and the enzymolysis process was determined by the activation rate of alcohol de-hydrogenase(ADH)after simulated gastrointestinal digestion.The highly anti-alcohol active components were ob-tained by ultrafiltration and ethanol dissolution,and the sobering effect was verified by animal experiments.The re-sults indicated that the wheat peptides prepared by trypsin enzymolysis had the most significant effect on ADH activa-tion and the optimal enzymolysis conditions obtained by response surface optimization include 0.5％of enzyme dosage(E/S),7.0 mg/mL of wheat gluten concentration,and 2.3 h of enzymolysis time.The component with molecular weight of 1-3 ku obtained by ultrafiltration separation had the highest ADH activation rate of 85.49％,and the acti-vation rate to ADH of the alcohol-soluble component obtained from further separation by 40％ethanol dissolution reached 120.3％.The results of righting reflex and open-field fine behavior indicated that the wheat gluten peptide had a certain effect on sobering up and alleviating the discomfort of mice after drinking alcohol.This study can pro-vide a reference for the preparation of wheat gluten anti-alcohol peptides.
wheat peptidesimulated gastrointestinal digestionethanol dehydrogenaseopen-field fine behavior
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