玉米肽螯合钙纳米粒的制备及结构表征分析
Preparation and Structural Characterization of Corn Peptide Chelated Calcium Nanoparticles
焦岩 1崔璐麟 2常影 2刘晓兰 1赵向宇 2张凯策 2林巍2
作者信息
- 1. 齐齐哈尔大学食品与生物工程学院,齐齐哈尔 161006;黑龙江省玉米深加工理论与技术重点实验室,齐齐哈尔 161006
- 2. 齐齐哈尔大学食品与生物工程学院,齐齐哈尔 161006
- 折叠
摘要
为了探明玉米肽与钙离子螯合纳米粒的结构与特性,以小分子玉米肽(CPT)为钙离子螯合载体,采用有机溶剂沉淀法制备玉米肽纳米螯合钙(CPT-Ca)复合纳米粒,研究了其制备工艺、结构表征及体外抗氧化活性.结果表明,玉米肽螯合钙(CPT-Ca)的最佳制备条件为:玉米肽与氯化钙质量比40∶1,pH 7.0,螯合时间为60 min,温度为50,此条件下肽钙螯合率为(81.20±3.55)％.该螯合钙粒子的平均粒径为(396.0±8.0)nm,多分散系数为0.043,Zeta电位为(-22.6±0.7)mV,具有良好的纳米粒子微观结构特性;傅里叶红外光谱(FTIR)、紫外吸收光谱(U V)和X射线衍射图谱(XRD)显示,玉米肽上的氨基与羧基提供结合位点以配位键方式与钙离子结合,形成新的肽-钙螯合物;抗氧化实验表明,螯合钙具有较好的羟基和DP-PH自由基清除自由基能力.玉米肽螯合钙纳米粒对于改善钙离子的结构分布和体内外传递吸收,开发新型玉米肽功能食品与钙离子补充剂具有重要作用.
Abstract
In order to reveal the structure and properties of corn peptide chelated calcium nanoparticles,the small molecule corn peptide(CPT)was used as a calcium ion carrier and corn peptide nano chelated calcium com-posite nanoparticles(CPT-Ca)were prepared by organic solvent precipitation method.The preparation process,structural characterization,and in vitro antioxidant activity of CPT-Ca were studied.The results indicated that the optimal preparation conditions of CPT-Ca were as follows:the mass ratio of corn peptide to calcium chloride was 40∶1,pH value was 7.0,chelation time was 60 minutes,temperature was 50℃,and the chelation rate of calcium peptide was(81.20±3.55)％under these conditions.The average particle size of the nano chelated calcium was(396.0±8.0)nm,the polydispersity coefficient was 0.043,and the Zeta potential was(-22.6±0.7)mV,exhibi-ting excellent nanoscale microstructure characteristics;Fourier transform infrared spectroscopy(FTIR),ultraviolet absorption spectroscopy(UV)and X-ray diffraction(XRD)revealed that the amino and carboxyl groups on the corn peptide provided a binding site to bind with calcium ions in the way of coordinate covalent bond,forming a new peptide calcium chelation;antioxidant experiments indicated that CPT-Ca had good hydroxyl and DPPH free radical scavenging ability.Corn peptide chelated calcium nanoparticles play an important role in improving the structural dis-tribution and in vivo and in vitro transmission and absorption of calcium ions,and in developing new corn peptide functional foods and calcium ion supplements.
关键词
玉米肽/纳米螯合钙/结构表征/抗氧化活性Key words
corn peptide/nano chelated calcium/structural characterization/antioxidant activity引用本文复制引用
基金项目
黑龙江省自然科学基金(LH2023C116)
黑龙江省省属高校基本科研业务费专项(145209144)
齐齐哈尔市科技计划市校合作项目(SXSP-2021001)
齐齐哈尔大学大学生创新创业训练计划(x202310232102)
出版年
2024
NETL
NSTL
万方数据