In this study,citrus microcarpa seeds were used as the raw materials,and citrus microcarpa seed oil was extracted through aqueous enzymatic method.The extraction process parameters were optimized,and the fatty acid composition and antioxidant activity were analyzed.Firstly,the extraction rate of citrus microcarpa seed oil was used as an indicator,the influence of single factor experiments(enzymatic hydrolysis time,enzymatic hydrolysis tem-perature,solid-liquid ratio,enzymatic hydrolysis pH value,and enzyme concentration)on the extraction rate of cit-rus microcarpa seed oil was investigated.On this basis,the response surface methodology was used to determine the optimal process conditions for enzymatic extraction of citrus microcarpa seed oil from effluent:enzymatic hydrolysis time of 12 hours,enzymatic hydrolysis temperature of 55 ℃,solid-liquid ratio of 1∶6.47,pH value of 3.30,and enzyme concentration of 4.47％.Secondly,a gas chromatograph was used to detect and analyze the fatty acid compo-sition and relative content of the obtained kumquat seed oil from the above processes.The results indicated that there were 8 kinds of fatty acids in the seed oil of citrus microcarpa,of which the relative content of Unsaturated fat acids could be as high as 67％.Finally,the antioxidant activity of citrus microcarpa seed oil was determined.The results in-dicated that citrus microcarpa seed oil had a certain reduction ability,with IC50 values of 0.76,2.55 and 0.23 mg/mL for DPPH·,O2-·,and·OH,respectively.
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