With rapeseed,sesame and flaxseed as the raw materials,the oil was produced by screw pressing,the vegetable oil DNA was extracted by emulsification,enrichment,CTAB,resin kit and freeze-drying methods,and the DNA quality was evaluated by ultra-microscopic visible spectrophotometry,real-time polymerase chain reaction(PCR),and microsatellite(SSR)molecular markers.The results indicated that the CTAB method extracts DNA with high concentration,good purity,short time consuming,low starting oil,low cost and suitable for molecular anal-ysis.The CTAB method was chosen to investigate the extraction efficiency of starting oil volume,organic compound addition,lysis buffer composition,lysis and precipitation time.Optimized conditions were a minimum starting oil vol-ume of 100 μL,organic compound addition:CTAB-chloroform:isoamyl alcohol(CTAB-C:I),lysis buffer com-position:CTAB-β mercaptoethanol-proteinase K(CTAB-βME-PK),lysis for 20 min and precipitation for 9 h.The method has extracted DNA suitable for molecular traceability in seven refined vegetable oils,providing technical support for traceability and authenticity testing of vegetable oils.
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