The Yangtze River Basin and the south of China are the best areas for Camellia oleifera planting,with the largest planting area in Hunan province.In order to explore the production adaptability of different Camellia oleif-era in Hunan,the differences of different Camellia oleifera varieties in Hunan were analyzed,and the effects of differ-ent varieties on the quality of Camellia oleifera seed oil were studied,including six main varieties in Hunan province:Huaxin,Huajin,Huashuo,Xianglin 210,Xianglin 1 and Xianglin 27,and other main planting varieties,such as Hengdong Datao series,Xianglin series,and Xianglin 97.The fatty acid composition and active substance content of different varieties of camellia seed oil were analyzed and evaluated by cluster analysis and principal component analy-sis.The results indicated that the main fatty acids of camellia seed oil were oleic acid,palmitic acid and linoleic acid.The unsaturated fatty acid content was more than 88%.The total content of sterols in Camellia oleifera seed oil was 583.35-1 623.79 mg/kg,and the main components were Δ7-stigmasterol and β-sitosterol,accounting for more than 85%of the total sterols.The main tocopherol in camellia seed oil was α-tocopherol,accounting for more than 80%of the total tocopherol.The content of squalene in camellia seed oil was 70.49-304.32 mg/kg.The poly-phenol content in oil-tea camellia seed oil was 3.37~19.14 mg/kg.The oil-tea camellia seed oil samples were divided into five categories by cluster analysis.The results of principal component analysis indicated that the compre-hensive score of Y15 ASUS oil-tea camellia seed oil was the highest.