玉米醇溶蛋白-槲皮素互作机制及其纳米颗粒的制备
Zein-Quercetin Nanoparticles:Interaction Mechanism and Preparation
王金霞 1王家薇 1王开俊 1吴长玲 1吴峰华 1刘兴泉 1王鹏1
作者信息
- 1. 浙江农林大学食品与健康学院,杭州 311300
- 折叠
摘要
以紫外光谱、荧光光谱和分子对接等方法探究玉米醇溶蛋白和槲皮素分子之间在醇溶体系内的相互作用机制,并通过粒径、包埋率及微观形貌等指标对其形成的纳米颗粒进行表征,初步探讨不同质量比的槲皮素与玉米醇溶蛋白对纳米颗粒的影响.结果表明,槲皮素与玉米醇溶蛋白发生了荧光静态猝灭,作用位点更接近玉米醇溶蛋白的色氨酸残基,其分子间相互作用力为氢键和范德华力.分子对接结果显示,槲皮素与玉米醇溶蛋白的氨基酸残基ALA-127、LEU-81、PRO-161、LEU-156形成疏水作用力,与SER-159和GLN-134残基形成氢键.玉米醇溶蛋白与槲皮素质量比为20:1时,形成了具有球状形貌核壳结构的纳米颗粒,且其粒径为88.31 nm,电位为35.96 mV,包埋率为81.09％.
Abstract
The UV spectrum,fluorescence spectrum,and molecular docking were used to investigate the interac-tion mechanism between quercetin and zein in ethanol-water solution.Additionally,the micro-morphology,en-trapment ratio,and particle size,etc.of the composite colloidal particles were determined to preliminary investigation how varied mass ratios of quercetin and maize gliadin affect nanoparticles.The findings revealed that both quercetin and zein demonstrated fluorescence static quenching,with zein's tryptophan residue serving as the action site.Van der Waals forces and hydrogen bonding were implicated in the intermolecular interaction.Molecular docking analysis indicated that quercetin formed hydrophobic interactions with amino acid residues ALA-127,LEU-81,PRO-161,and LEU-156 of zein,as well as hydrogen bonds with residues SER-159 and GLN-134.When the mass ra-tio of zein to quercetin was 20:1,core-shell nanoparticles were formed,characterized by a particle size of 88.31 nm,a potential of 35.96 mV,and an embedding rate of 81.09％.
关键词
玉米醇溶蛋白/槲皮素/互作机制/纳米颗粒Key words
zein/quercetin/interaction mechanism/nanoparticles引用本文复制引用
出版年
2024
NETL
NSTL
万方数据